重铬酸钾对卵黄囊细胞膜脂质流动性的影响  

Effects of Potassium Dichromate on Lipid Fluidity of Cell Membrane of Yolk Sac in Rats

作　　者：袁立懋 孙棉龄[3] 吴德生[3] 张本忠[4] 

机构地区：[1]华西医科大学 [2]广州市卫生防疫站,广州510080 [3]华西医科大学公共卫生学院 [4]兰州医学院预防医学系

出　　处：《环境与健康杂志》1999年第5期272-273,共2页Journal of Environment and Health

基　　金：国家教委博士点基金

摘　　要：为探讨重铬酸钾导致卵黄囊功能紊乱的机理，以１，６二苯１，３，５已三烯（ Ｄ Ｐ Ｈ）为荧光探针，用荧光偏振技术观察重铬酸钾对大鼠卵黄囊细胞膜脂质流动性的影响。结果表明：重铬酸钾浓度为１０ ｍ ｇ／ Ｌ以上时，卵黄囊细胞膜脂质流动度（ Ｌ Ｆ Ｕ）降低（ Ｐ＜ ００５），胚胎发育受到抑制（ Ｐ＜ ００５），且都呈明显的剂量效应关系。提示卵黄囊细胞膜脂质流动度改变可能是重铬酸钾对大鼠胚胎发育毒性的机理之一。To explore the mechanism of dysfunction of yolk sac induced by potassium dichromate (K 2Cr 2O 7),the variances of lipid fluidity of cell membrane of yolk sac induced by K 2Cr 2O 7 were determined by fluorescence polarization technique using 1,6 diphenyl 1,3,5 hexatrifiene (DPH) probe in rats in vitro The results showed that the lipid fluidity of cell membrane of yolk sac decreased significantly ( P <0 05),and embryonic development was inhibited with the concentrations of K 2Cr 2O 7,which were more than 1 0 mg/L Significant dose effect relationships were observed in those two indexes mentioned above It indicated that the variances of lipid fluidity of cell membrane of yolk sac might be one of the mechanisms for the demage of embryonic development induced by K 2Cr 2O 7 in rats

关 键 词：重铬酸钾 卵黄囊 生物膜 脂质流动性 

分 类 号：R595.9[医药卫生—内科学]