不同浓度VEGF对AECII表达SP-B和TGF-β1的影响及意义  

Roles of VEGF in the expressions of SP-B and TGF-β1 in type 2 alveolar epithelial cells

作　　者：薛春红[1] 曾万江[1] 乔福元[1] 刘海意[1] 祝秀[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《中国优生与遗传杂志》2011年第4期20-21,38,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)对肺泡II型细胞(type 2 alveolar epi-thelial cell,AECⅡ)表面活性蛋白-B(surfactant associated protein,SP-B)和转化生长因子-β1(transforming growth fac-tor beta-1,TGF-β1)表达的影响及意义。方法原代培养早产鼠AECII,采用real-time RT-PCR、细胞免疫染色等方法,观察VEGF对AECII SP-B及TGF-β1表达的影响。结果 Real-time PCR结果显示25ng/ml VEGF组与正常组相比SP-B mRNA表达水平明显上升(P<0.05)。25ng/ml VEGF组与正常组相比TGF-β1 mRNA表达明显下降(P<0.05),其他各组与正常组相比无统计学差异(P>0.05)。结论 VEGF在适宜浓度时有促进SP-B表达的作用,同时有抑制TGF-β1表达的作用,推测VEGF促进SP-B表达的作用与直接或间接抑制TGF-β1的表达有关。Objective：To analyze the effects and the significances of VEGF on the mRNA expressions of the SP-B and TGF-β1 of AECⅡs.Methods：AECⅡs were isolated and purified from the fetal rat lung tissues,then cultured with different concentrations of VEGF.Observe the effects of VEGF on mRNA levels of SP-B and TGF-β1 using real-time RT-PCR and Immunocytochemistry.Results：25ng /ml VEGF can stimulate the expression of SP-B on mRNA levels significantly（P0.05）.And 25ng/ml VEGF can inhibit the mRNA expression of TGF-β1 significantly（P0.05）,other groups have no significant change（P 0.05）.Conclusion：Both of VEGF can stimulate the expression of SP-B on mRNA levels significantly at the appropriate concentration.When it stimulated the expression of SP-B,the expression of TGF-β1 was inhibited significantly（P0.05）.We speculate that VEGF stimulated the mRNA expression of SP-B by inhibiting expression of TGF-β1 directly or indirectly.

关 键 词：血管内皮细胞生长因子 肺泡Ⅱ型细胞 表面活性蛋白-B 转化生长因子 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]