新生大鼠缺氧缺血损伤后心肌细胞GAP-43的表达  

Expression of growth-associated protein in myocardium of neonatal rats with hypoxic-ischemic damage

作　　者：姚笠[1] 刘影[1] 程琳[2] 付仲秋[1] 赵霞霞[1] 

机构地区：[1]哈尔滨医科大学第二临床医学院儿内科,150086 [2]哈尔滨医科大学组织学与胚胎学教研室

出　　处：《中国优生与遗传杂志》2011年第4期24-25,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究生长相关蛋白-43(growth associated protein-43,GAP-43)在新生鼠缺氧缺血损伤后心肌细胞中的差异表达及其临床意义。方法取42只7日龄Wistar大鼠随机分为正常对照组和缺氧缺血组。大鼠分别于缺氧缺血后2d,3d,7d断头处死取心肌,应用免疫组化法检测心肌细胞上GAP-43的表达。结果缺氧缺血组GAP-43的表达较对照组明显增加,在缺氧缺血后2d至7d差异有显著意义(P<0.05)。结论缺氧缺血大鼠心肌GAP-43表达呈时间依赖性变化,与心肌细胞损害密切相关。Objective：To investigate the expression and the clinical significance of expression of growth-associated protein in myocardium of neonatal rats with hypoxic-ischemic damage.Methods：Twenty one 7-day old Wistar rats were used normal group,and another twenty one 7-day-old Wistar rats with hypoxic-ischemic damage.Immunohistohippocampus was respectively used to detect the expression of GAP-43 protein in all rats hippocampus on day 2,3,7 after hypoxic-ischemic damage.Results：The expression of GAP-43 protein was obviously increased in the rats with hypoxic-ischemic damage as compared with normal rats and from 2d to7d after hypoxic-ischemic damage had significant differences（P0.05）.Conclusions：The expression of GAP-43 in myocardium of neonatal rats may change with age.It is related to myocardium injury.

关 键 词：缺氧缺血 GAP-43 新生大鼠 

分 类 号：R722.1[医药卫生—儿科]