卵巢早衰microRNA的差异表达及其作用  

MicroRNA expression profiles in premature ovarian failure patients and its potential regulate functions

作　　者：周莹[1] 朱英哲[1] 张素华[1] 王红梅[2] 王树玉[1] 杨晓葵[1] 

机构地区：[1]首都医科大学附属北京妇产医院生殖医学科,北京100026 [2]中国科学院动物所生殖生物学国家重点实验室,北京100101

出　　处：《中国优生与遗传杂志》2011年第5期20-22,共3页Chinese Journal of Birth Health & Heredity

基　　金：国家人事部留学人员科技活动择优资助项目;北京市优秀人才基金资助(20061D0301000091);北京市卫生局"十百千"项目

摘　　要：目的探讨卵巢早衰患者(POF)与正常人血浆microRNA(miRNA)表达差异,研究差异miRNA表达与卵巢早衰的关系。方法应用microarray芯片技术检测卵巢早衰患者和正常人血浆中的miRNA表达差异,实时定量PCR(qRT-PCR)验证从microarray芯片中筛选出来的明显差异表达的miRNA。结果 microarray芯片技术结果显示,与正常组比较,卵巢早衰患者的血浆中10种miRNA表达显著上调:mir-202、mir-146a、mir-125b-2、mir-139-3p、mir-654-5p、mir-27a、mir-765、mir-23a、mir-342-3p及mir-126,2种miRNA表达显著下调:let-7c和mir-144。结论卵巢早衰患者和正常人血浆中miRNAs表达谱有差异,这种差异可能与POF发病有关。Objective：To detect the differentially expressed microRNAs（miRNA）from the serum of premature ovarian failure（POF）patients and normal women,and explore the effects of microRNA on POF.Methods：An oligonucleotide microarray chip was used to profile miRNA expressions in POF patients and normal women.The significantly different miRNA,which was selected from microarray chip,validated by quantitative real-time PCR（qRT-PCR）.Results：The microarray chip results demonstrated that 10 miRNAs（mir-202,mir-146a,mir-125b-2,mir-139-3p,mir-654-5p,mir-27a,mir-765,mir-23a,mir-342-3p及mir-126） were significantly upregulation and 2 miRNAs（let-7c and mir-144） downregulation in POF patients compared with normal women.Among these miRNA,overexpression of mir-146a,mir-23a,mir-27a and mir-126 were confirmed by qRT-PCR.Conclusions：Some miRNAs expressed differently between POF patients and normal women,suggesting that they may be involved in the pathogensis of POF.

关 键 词：MIRNA 卵巢早衰 颗粒细胞 凋亡 

分 类 号：R711.75[医药卫生—妇产科学]