流式细胞术检测HLA-B27及其临床意义  

Detection of HLA-B27 by flow cytometry and it′s clinical evaluation

作　　者：金明威 陈哲 陈颖 

机构地区：[1]宁波鄞州人民医院血液病实验室,浙江宁波315040

出　　处：《中国优生与遗传杂志》2011年第5期35-35,32,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的通过流式细胞术检测外周血白细胞HLA-B27的表达,并探讨其对强直性脊柱炎(AS)的诊断价值。方法利用流式细胞仪(FCM)检测41例AS患者、62例背痛及腰腿痛患者及112例健康体检者的外周血HLA-B27表达。结果 41例AS患者中,HLA阳性38例(92.6%);62例背痛腰腿痛患者中,HLA-B27阳性6例(9.67%);112例健康人群中,HLA-B27阳性2例(1.78%)。结论 AS患者HLA-B27阳性率明显增高,HLA-B27检测可视为临床支持AS诊断的重要依据。Objective：To study the clinical evaluation of HLA-B27 detection by flow cytometry（FCM） for diagnosing ankylostomiasis.Methods：41 patients with AS,62 patients with lumbago and 112 normal people were analyzed by FCM.Results：HLA-B27 were positive in 38（92.6%）;among 41 patients with AS,6（9.67%） of 62 patients with lumbago and 2（1.78%） of 112 normal persons.Conclusions：The positive rate of HLA-B27 in patients with AS is obviously higher than that of other groups.The detection of HLA-B27 by FCM can play an important role in diagnosing AS.

关 键 词：流式细胞术 强直性脊柱炎 HLA-B27 

分 类 号：R440[医药卫生—诊断学]