人脐带干细胞携带NGF基因脑内移植对脑损伤大鼠神经行为学的影响  

Transplantation of NGF modified umbilical cord mesenchymal stem cells improves neurological deficits in rats subjected to brain trauma

作　　者：袁源[1] 但齐琴[2] 刘佳[3] 

机构地区：[1]烟台毓璜顶医院神经外科,烟台264005 [2]四川大学华西医院神经疾病研究所 [3]昆明医学院神经科学研究所

出　　处：《中华行为医学与脑科学杂志》2011年第4期298-301,共4页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：基金项目：烟台市卫生局资助项目（2007-139-4）;美国中华医学基金项目（CNB00-72）

摘　　要：目的 探讨人脐带间充质干细胞（UCMSC）携带NGF基因脑内移植对脑损伤大鼠神经行为学恢复的影响.方法 建立大鼠自由落体颅脑损伤动物模型;培养纯化人脐带干细胞（UCMSC）,构建NGF-pcDNA III重组质粒载体,并转染UCMSC;体外检测UCMSC携带NGF基因的表达,并进行生物活性分析.将成功表达NGF的UCMSC局部移植入大鼠损伤脑组织.2周进行神经功能缺损评分（NSS）后,取脑组织行NGF免疫组织化学染色检测NGF表达情况.结果 脑损伤大鼠呈现明显的神经功能障碍（10.50±0.53）分;移植UCMS在损伤区存活,迁移,并促进大鼠神经功能部分恢复（7.75±0.71）分;而携带NGF基因的UCMSC移植导致神经功能明显改善（5.38±0.52）分,与单纯UCMSC移植组比较,差异有统计学意义（P＜0.01）.结论 UCMSC作为携带NGF基因的细胞载体可用于脑内移植,NGF转基因UCMSC移植对脑损伤大鼠神经行为学恢复有明显的促进作用.Objective To investigate the effect of NGF gene modified umbilical cord mesenchymal stem cells （UCMSC） transplantation on neurological functional improvement in traumatic brain rats. Methods Cerebral contusion model in motor-sensory cortex in rats was established by a weight hammer falling method. UCMSC were culutred and transferred with NGF gene. After NGF expression and activity was identified,the NGF gene modified UCMSC were engrafted into injured brain. The neurological function was evaluated 2 weeks after brain injury. And the NGF immunostaining was also performed to explore the level of NGF expression. Results Severe neurological dysfunction（ 10.50 ± 0.53 ）occurred in rats after traumatic brain injury, while the UCMSC transplantaion led to a significant functional improvement（ 7.75 ± 0. 71 ）（P 〈 0. 01 ）. Moreover, the best functional improvement was found in rats receiveing UCMSC grafts modified with NGF gene （5.38 ± 0. 52 ） （P 〈 0.01 ）. Conclusion NGF gene modified UCMSC transplantation can improve neural behavior in rats with brain trauma.

关 键 词：脑损伤 脐带干细胞 神经功能评价 神经生长因子 大鼠 

分 类 号：R651.1[医药卫生—外科学]