In vivo perfusion staining of atherosclerotic lesions and a novel quantification method for lesion size in sequential aortic root sectioning in murine models  

作　　者：QIU Ying Mark M. Yun WANG Yi-zhong SU Wu-yun MENG Xing-kai YUN Sheng 

机构地区：[1]Department of Clinical Sciences, Ryne Institute, King's Col- lege London, UK. [2]The First Teaching Hospital, Inner Mongolia Medical College, Huhhot, China. [3]UCL Medical School, University College London, UK. [4]The Aerspace Medical School, Peking University. [5]Hammersmith Campus, Imperial College London, Du Cane Road, London W12 0NN, UK

出　　处：《South China Journal of Cardiology》2011年第1期40-48,55,共10页岭南心血管病杂志（英文版）

基　　金：supported by the Hong Kong WangKuan Cheng Foundation Grant;British Heart Foundation

摘　　要：Objectives To develop a simple, accurate and reproducible method, which combines macro and histopathological techniques for determining the degree of lipid deposition in genetically modified mice. Method The entire aortas from C57BL/6, ldlr-/- and apoE-/- mice were stained with Sudan IV using either in vivo perfusion or traditional in vitro enface staining techniques. Histological sections of aortic root and hearts were embedded in tissue freezing medium and cut with a cryostat, then stained with Oil Red O. The calculated aortic root area based on the aortic root circumference was used to reduce measurement errors. Results The in vitro en face staining can stain all fat, which include the adventitial tissue around aorta. However the in vivo perfusion staining can specifically stain the fatty deposition inside of aorta. Both entire aorta and aortic root section staining showed that there was a highly significant increase in fatty deposition in the aortas of the genetic modified mice. Although all mice genetic background was same, the apoE-/- mice had larger atherosclerotic lesions than ldlr-/- mice. Conclusions The new in vivo peffusion method is more accurate than the in vitro en face method. The combination of these macro and microscopic techniques overcomes the shortcomings of the earlier published methods which are generally limited to the measurement of fatty red staining areas only, neglecting non-specific adventitial fat staining around aorta and aortic root section tissue distortion.Objectives To develop a simple, accurate and reproducible method, which combines macro and histopathological techniques for determining the degree of lipid deposition in genetically modified mice. Method The entire aortas from C57BL/6, ldlr-/- and apoE-/- mice were stained with Sudan IV using either in vivo perfusion or traditional in vitro enface staining techniques. Histological sections of aortic root and hearts were embedded in tissue freezing medium and cut with a cryostat, then stained with Oil Red O. The calculated aortic root area based on the aortic root circumference was used to reduce measurement errors. Results The in vitro en face staining can stain all fat, which include the adventitial tissue around aorta. However the in vivo perfusion staining can specifically stain the fatty deposition inside of aorta. Both entire aorta and aortic root section staining showed that there was a highly significant increase in fatty deposition in the aortas of the genetic modified mice. Although all mice genetic background was same, the apoE-/- mice had larger atherosclerotic lesions than ldlr-/- mice. Conclusions The new in vivo peffusion method is more accurate than the in vitro en face method. The combination of these macro and microscopic techniques overcomes the shortcomings of the earlier published methods which are generally limited to the measurement of fatty red staining areas only, neglecting non-specific adventitial fat staining around aorta and aortic root section tissue distortion.

关 键 词：in vivo peffusion staining in vitro en face staining lesion quantification atherosclerotic lesion ldlr-/- apoE-/- 

分 类 号：TS193[轻工技术与工程—纺织化学与染整工程] Q513.5[轻工技术与工程—纺织科学与工程]