筛选蛋白质结晶条件的试剂盒研发进展  

Progresses on Developing Screening Kits for Protein Crystallization

作　　者：张仙芳[1] 刘君[1] 郭云珠[1] 马晓亮[1] 尹大川[1] 

机构地区：[1]西北工业大学生命学院空间生物实验模拟技术国防重点学科实验室,西安710072

出　　处：《生物化学与生物物理进展》2011年第3期280-287,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(10772150)~~

摘　　要：蛋白质结晶条件筛选是蛋白质分子三维结构解析的主要限速环节之一,因此发展筛选效率较高的结晶条件筛选试剂盒有着十分重要的意义.目前广泛应用的结晶条件筛选试剂盒的设计方法主要有不完全因子法、稀疏矩阵法和系统筛选法.结晶学家基于这三种方法研发了一系列用于蛋白质结晶条件筛选的试剂盒.评述了20世纪以来筛选试剂盒的设计方法及应用进展,并展望了其未来的发展方向.Screening of protein crystallization conditions remains the major limiting step for three-dimensional structure determination, therefore developing more efficient screening kits is essential. Up to now the methods used for designing the screening kits include the incomplete factorial design, the sparse matrix sampling and the systematic screening. Based on these three methods, researchers have developed a series of crystallization screening kits which are proved applicable in practical protein crystallization. The development in designing the screening kits and the applications of the screening kits since 20th century were reviewed, and the future prospect in this field was discussed.

关 键 词：蛋白质结晶 筛选试剂盒 不完全因子 稀疏矩阵 系统筛选 

分 类 号：Q617[生物学—生物物理学]