益气血补肝肾中药对体外受精-胚胎移植周期中卵泡液转化生长因子β_1和性激素水平的影响  

Effect of Chinese Drugs for Supplementing Qi-blood and Nourishing Gan-Shen on Transforming Growth Factor β_1 and Sex Hormone Levels in the Follicle Fluid of Women during IVF-ET Cycle

作　　者：葛明晓[1] 张金玉[1] 赵彦鹏[1] 林德伟[1] 邓伟民[1] 

机构地区：[1]广州军区广州总医院妇产科辅助生育中心,广州510010

出　　处：《中国中西医结合杂志》2011年第3期327-330,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省中医药局科研课题(No.2009437)

摘　　要：目的研究体外受精-胚胎移植(IVF-ET)周期加用益气血补肝肾中药对卵泡液转化生长因子β1(TGFβ1)和性激素水平的影响。方法 行IVF-ET患者86例,随机分成中药组(45例)及对照组(41例),中药组于促性腺激素释放激素激动剂(GnRH-a)降调节同时口服中药促黄体颗粒1周,给予促性腺激素(Gn)促排卵时口服中药经后增殖颗粒至人绒毛膜促性腺激素(HCG)日;对照组采用标准方案促排卵治疗不加用中药,两组均于取卵日留卵泡液,用酶联免疫反应法(ELISA)测定TGFβ1,放射免疫法测定雌二醇(E2)及孕酮(P)和促黄体生成素(LH)水平。结果 中药组卵泡液TGFβ1[(3.25±1.11)pg/L]和LH水平[(0.89±0.45)IU/L]明显高于对照组[(2.21±1.08)pg/L,(0.57±0.42)IU/L,P<0.05]。结论 益气血补肝肾法能显著提高卵泡液TGFβ1和LH水平,从而提高胚胎的种植率。Objective To study the effect of Chinese drugs for supplementing qi-blood and nourishing Gan-Shen（CD） on transforming growth factor β1（TGF β1） and sex hormone levels in the follicle fluid of women during in vitro fertilization-embryo transfer（IVF-ET） cycle.Methods Eighty-six women undergoing IVF-ET were randomly assigned to two groups,45 in the CD group and 41 in the control group.All received the standard regimen for promoting ovulation,but to women in the CD group,1-week treatment of Cuhuangti Granule was administered during the period of gonadotropin releasing hormone agonist（GnRH-a） down-regulation,and Jinghou Zengzhi Granule was given from time of ovulation promoting with Gn to the day of HCG administration.On the day of oocyte retrieval,TGFβ1 was detected using enzyme linked immunosorbent assay（ELISA）,estrradiol（E2）,progestone（P）,and luteinizing hormone（LH） detected by radioimmunoassay.Results Follicle fluid contents of TGFβ1 and LH in the CM group（3.25±1.11 pg/L and 0.89±0.45 IU/L） were obviously higher than those in the control group（2.21±1.08 pg/L and 0.57±0.42 IU/L,both P0.05）.Conclusion Chinese drugs for supplementing qi-blood and nourishing Gan-Shen could significantly improve TGFβ1 and LH levels in the follicle fluid of women,thus enhancing the embryo implantation rate.

关 键 词：益气血补肝肾 体外受精-胚胎移植 转化生长因子β1 促黄体生成素 

分 类 号：R714.8[医药卫生—妇产科学]