益气补肾中药对自然流产模型小鼠脾脏和母胎界面CD4^+CD25^+T细胞表达的影响  

Effect of Chinese Drugs for Invigorating Qi and Tonifying Shen on Expression of CD4^+CD25^+ Regulatory T Cells in Spleen and Maternal-fetal Interface of Abortion-prone Mice

作　　者：叶平[1,2] 胡蓝雅文 黄丹云[1] 丁键红[1] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310053 [2]浙江省中医院,杭州310006 [3]杭州市江干区采荷街道社区卫生服务中心,杭州310016

出　　处：《中国中西医结合杂志》2011年第3期359-362,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：浙江省教育厅资助项目(No.20071142)

摘　　要：目的观察益气补肾中药对自然流产模型小鼠脾脏和母胎界面CD4+CD25+T淋巴细胞表达的影响。方法 将雌性CBA与雄性DBA/2小鼠2:1合笼交配,造自然流产模型。将CBA孕鼠随机分为4组:阴性对照组(NS组)、阳性对照组(CsA组)、中药组、中西组(中药加CsA联合干预组)。将孕鼠分为两批,分别于妊娠第9、14天将其处死,取脾脏、蜕膜组织,采用流式细胞术分析CD4+CD25+T细胞的表达情况。结果 (1)孕9天时,与阴性对照组比较,各治疗组脾脏CD4+CD25+T细胞的表达均明显升高,有统计学意义(P<0.01,P<0.05),各治疗组间比较差异无统计学意义(P>0.05);孕14天时,与阴性对照组比较,各治疗组脾脏CD4+CD25+T细胞的表达均明显升高,有统计学意义(P<0.01,P<0.05),其中,中西组CD4+CD25+T细胞的表达最高,与中药组及CsA组比较,差异有统计学意义(P<0.01),中药组与CsA组之间比较差异无统计学意义(P>0.05)。(2)孕9天时,与阴性对照组比较,各治疗组母胎界面CD4+CD25+细胞表达在数值上均有升高,但无统计学意义(P>0.05)。从趋势上看,各组按CD4+CD25+T细胞表达由高到低排序,依次为:中西组、CsA组、中药组、阴性对照组;孕14天时,与阴性对照组相比,中西组CD4+CD25+T细胞在母胎界面的表达明显升高,有统计学意义(P<0.05),中药组及CsA组CD4+CD25+T细胞的表达也较阴性对照升高,但无统计学意义(P>0.05)。结论 益气补肾中药可上调自然流产模型小鼠孕早期及中晚期脾脏CD4+CD25+T细胞表达,益气补肾中药联合CsA干预可上调自然流产模型小鼠孕中晚期母胎界面CD4+CD25+T细胞的表达,提示益气补肾中药有助于妊娠时免疫耐受状态的维持。Objective To study the effect of Chinese drugs for invigorating qi and tonifying Shen（IQTS） on expression of CD4＋CD25＋ regulatory T cells in spleen and maternal-fetal interface of abortion-prone mice during pregnancy.Methods CBA female mice were mated with DBA/2 male mice to establish abortion-prone models,which were randomly divided into 4 groups,the negative control group（fed with normal saline）,the positive control group（treated with CsA）,the Chinese medicine group（treated with IQTS）,and the Chinese and Western medicine group（treated with IQTS＋CsA）.Mice were sacrificed in batches on the 9th and the 14th day of gestation,their splenic and decidual tissues were taken out to analyse CD4＋CD25＋regulatory T-cell expression by flow cytometry.Results Compared with the negative control group,the expression of splenic CD4＋CD25＋regulatory T all significantly increased on the 9th day of gestation（P0.01 or P0.05）.There was no statistical difference in inter-group comparison of the three treatment groups（P0.05）.Compared with the negative control group,the expression of splenic CD4＋CD25＋regulatory T all significantly increased on the 14th day of gestation（P0.01 or P0.05）.Of them,its expression was the highest in the Chinese and Western medicine group,showing significant difference from that in the Chinese medicine group and the positive group（P0.01）.The difference between the Chinese medicine group and the positive group was insignificant（P0.05）.On day 9 of gestation,compared with the negative control group,the expressions of CD4＋CD25＋ regulatory T in maternal-fetal interface increased in the three treated groups,showing no statistical significance（P0.05）.Its expression was ordered from high to low in sequence as the Chinese and Western medicine group,the positive control group,the Chinese medicine group,and the negative control group.On day 14 its expression was obviously enhanced in the Chinese and Western medicine group,showing statistical difference from t

关 键 词：小鼠 自然流产模型 CD4+CD25+T细胞 益气补肾中药 

分 类 号：R285.5[医药卫生—中药学]