毛细支气管炎患儿红细胞免疫及T细胞亚群变化的研究  

Changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis

作　　者：刘义红[1] 熊丽萍[1] 沈红元[2] 

机构地区：[1]华中科技大学同济医学院附属孝感医院儿科,湖北孝感432000 [2]湖北职业技术学院医学院检验中心,湖北孝感432000

出　　处：《中国当代儿科杂志》2011年第3期202-204,共3页Chinese Journal of Contemporary Pediatrics

摘　　要：目的探讨毛细支气管炎患儿红细胞免疫和T细胞亚群的变化及意义。方法对45例毛细支气管炎患儿和30例正常儿童的红细胞免疫功能和T细胞亚群进行检测。检测外周血红细胞免疫复合物花环率(RBC-ICR)、红细胞C3b受体花环率(RBC-C3bRR);采用流式细胞术检测CD3+、CD4+、CD8+细胞亚群。结果毛细支气管炎患儿RBC-C3bRR[(13.6±6.2)%]、CD8+细胞百分比[(21.6±4.4)%]较对照组的(18.0±7.4)%和(25.6±5.2)%减低(P<0.01);CD3+[(59.9±6.7)%]和CD4+细胞百分比[(53.5±6.2)%]及RBC-ICR[(8.3±3.5)%]均高于对照组的(52.1±8.3)%、(46.8±4.9)%和(6.1±2.5)%(P<0.01)。毛细支气管炎患儿RBC-ICR和CD4+细胞百分比存在正相关(r=0.63,P<0.05),RBC-C3bRR和CD4+细胞百分比存在负相关(r=-0.82,P<0.01)。结论毛细支气管炎患儿存在T淋巴细胞、红细胞免疫功能障碍,可能在毛细支气管炎的发病机制中起到一定作用。Objective To study the changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis and their possible roles in the pathogenesis of bronchiolitis. Methods Forty-five children with bronchiolitis and 30 healthy controls were enrolled.Red cell immune complex rosette（RBC-ICR） and red cell C3b receptor rosette（RBC-C3bRR） were detected.The percentages of CD3^＋,CD4^＋ and CD8＋ cells were assayed by flow cytometry. Results RBC-C3bRR [（13.6±6.2）% vs（18.0±7.4）%] and the percentage of CD8^＋ cells [（21.6±4.4）% vs（25.6±5.2） %] in the bronchiolitis group were lower than those in the control group（P〈0.01）.The percentage of CD3^＋ cells [（59.9±6.7）% vs（52.1±8.3）%] and CD4^＋ cells [（53.5±6.2）% vs（46.8±4.9）%] and RBC-ICR [（8.3±3.5）% vs（6.1±2.5）%] in the bronchiolitis group were higher than those in the control group（P〈0.01）.The percentage of CD4＋ cells was positively correlated with RBC-ICR（r=0.63,P〈0.05） and negatively correlated with RBC-C3bRR（r=-0.82,P〈0.01）. Conclusions There are dysfunctions of red cell immune and T-lymphocyte subsets in children with brochiolitis,which may play a role in the pathogenesis of brochiolitis.

关 键 词：毛细支气管炎 T淋巴细胞 红细胞免疫 儿童 

分 类 号：R725.6[医药卫生—儿科]