地塞米松对急性哮喘大鼠肺组织15-脂氧合酶表达的影响  

Effects of dexamethasone on 15-lipoxygenase expression in lungs of asthmatic rats

作　　者：革丽莎[1] 李昌崇[1] 张维溪[1] 梁亚峰[1] 张和[1] 

机构地区：[1]温州医学院附属育英儿童医院呼吸科,浙江温州325027

出　　处：《中国当代儿科杂志》2011年第3期227-230,共4页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金资助项目(No.30973232)

摘　　要：目的 15-脂氧合酶(15-LO)是一种表达于哮喘肺组织,参与抗炎和促炎物质生成的过氧化物酶。基因队列研究认为它可能对过敏性哮喘起保护作用。本研究通过观察哮喘大鼠肺组织15-LO的表达以及地塞米松对其表达的影响,探讨15-LO在哮喘中的作用。方法将27只雄性Sprague-Dawley大鼠随机分为:正常对照组、哮喘组、地塞米松组。采用卵清白蛋白(OVA)致敏、激发,建立支气管哮喘大鼠气道炎症模型。ELISA法测定肺组织匀浆15-LO蛋白含量;RT-PCR法检测肺组织15-LO mRNA表达水平。结果哮喘组大鼠肺组织匀浆15-LO蛋白含量(2080±73μg/mL)及肺组织15-LO mRNA转录水平(0.51±0.14)均显著低于正常对照组的(2472±106μg/mL)和(0.76±0.15)(P<0.01);地塞米松干预后15-LO蛋白(2562±218μg/mL)及mRNA(1.02±0.34)表达均上调(P<0.01)。结论哮喘大鼠肺组织15-LO表达低下,地塞米松可能部分通过上调哮喘大鼠肺组织15-LO而发挥抗炎作用。Objective 15-lipoxygenase（15-LO） is a prooxidant enzyme which is expressed in asthmatic lungs leading to formation of pro-and anti-inflammatory mediators.Gene expression profiling studies show the association between 15-LO and allergic asthma.This study was designed to observe the expression of 15-LO in lungs of asthmatic rats and examine the effects of dexamethasone on 15-lipoxygenase expression. Methods Twenty-seven male Sprague-Dawley（SD） rats were randomly divided into three groups： control,asthma and dexamethasone（DXM） intervention.An asthma model was prepared by sensitization and challenging with ovalbumin.The production of 15-LO in lung tissue homogenates was measured using ELISA.The expression of 15-LO mRNA in lungs was determined by reverse transcription-polymerase chain reaction（RT-PCR）. Results The levels of 15-LO mRNA and protein in the asthma group（0.51±0.14 and 2080±73 μg/mL,respectively） were lower than those in the control group（0.76±0.15 and 2472±106 μg/mL,respectively;P〈0.01）.DXM intervention increased significantly the levels of 15-LO mRNA and protein（1.02±0.34 and 2562±218 μg/mL） compared with the asthma group（P〈0.01）. Conclusions The production of 15-LO in lung tissues is reduced in asthmatic rats.DXM can increase the expression of 15-LO in lung tissues and thus might provide anti-inflammatory effects in asthmatic rats.

关 键 词：15-脂氧合酶 支气管哮喘 炎症 地塞米松 大鼠 

分 类 号：R562.25[医药卫生—呼吸系统] R965[医药卫生—内科学]