依达拉奉对惊厥持续状态幼年大鼠海马GFAP和IL-lβ表达及细胞凋亡的影响  

Effects of edravone on glial fibrillary acidic protein and interleukin-lβ expression and neuronal apoptosis in juvenile rat hippocampus after status convulsion

作　　者：王海萍[1,2] 邓小龙[3] 李光乾[1,2] 

机构地区：[1]浙江中医药大学附属杭州市第六医院 [2]杭州市儿童医院神经科,浙江杭州310014 [3]武汉市儿童医院神经科,湖北武汉430016

出　　处：《中国当代儿科杂志》2011年第3期231-235,共5页Chinese Journal of Contemporary Pediatrics

摘　　要：目的观察幼年大鼠惊厥持续状态(SC)后海马胶质纤维酸性蛋白(GFAP)、白介素-lβ(IL-lβ)的表达及神经细胞凋亡的变化,并探讨依达拉奉(ED)对三者的影响。方法将195只Sprague-Dawley幼年雄性大鼠随机分为生理盐水对照组(NS组)、惊厥持续状态组(SC组)和依达拉奉干预组(ED组),每组65只,各组均按SC后处死时间分为4 h、12 h、24 h、48 h、72 h 5个亚组,每组13只。采用氯化锂-匹鲁卡品制备幼年大鼠SC模型。应用逆转录多聚酶链反应(RT-PCR)法检测海马GFAP mRNA表达,免疫组化法检测海马中GFAP、IL-lβ蛋白表达,TUNEL法检测神经细胞凋亡。结果 (1)免疫组化结果显示SC组海马中GFAP和IL-1β表达增强,与NS组比较差异有统计学意义;与SC组比较,ED组GFAP和IL-1β表达明显降低,差异有统计学意义;(2)RT-PCR法检测结果显示GFAP表达趋势与蛋白基本相似;(3)SC组在惊厥12 h时间点海马CA1区TUNEL阳性细胞数已显著高于NS组,48 h达高峰,而ED组TUNEL阳性细胞数在12～48 h时间点均较SC组显著下降,但仍高于NS组。结论 SC后大鼠海马GFAP和IL-1β的表达增强,ED可下调SC大鼠海马GFAP和IL-1β的表达,并使神经细胞凋亡减少;提示ED对SC引起的脑损伤可能有保护作用。Objective To study the effects of edaravone on glial fibrillary acidic protein（GFAP） and interleukin-1β（IL-lβ） expression and neuronal apoptosis in the juvenile rat hippocampus after status convulsion（SC）. Methods One hundred and ninety-five juvenile male Sprague-Dawley（SD） rats were randomly divided into 3 groups： normal saline control and SC with and without edaravone treatment.Each of the 3 groups was further subdivided into subgroups sacrificed at 4,12,24,48 and 72 hrs after SC（n=15）.The SC model was prepared using lithium-pilocarpine.The expression of GFAP and IL-lβ protein was detected with immunohistochemistry methods.The neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling（TUNEL）.The hippocampal GFAP mRNA expression was detected by RT-PCR. Results The value of IOD of GFAP and IL-lβ positive cells measured by immunohistochemistry in the untreated SC group increased compared with the control group.Expression of GFAP and IL-lβ protein was significantly reduced in the edaravone treated SC group compared with the untreated SC group.RT-PCR showed the expression trend of GFAP mRNA was similar to that of protein.The TUNEL positive cells in the hippocampus CA1 in the untreated SC group increased significantly 12 hrs after SC and reached a peak at 48 hrs compared with the control group.The intervention with edaravone decreased significantly TUNEL positive cells between 12-48 hrs after SC,but the number of TUNEL positive cells in the intervention group remained significantly greater than in the control group. Conclusions The expression of GFAP and IL-lβ in the hippocampus increases after SC in rats.Edaravone may decrease the expression of GFAP and IL-1β and reduce the number of neuronal apoptosis.These results suggest that edaravone may have protective effects against brain damage caused by SC.

关 键 词：惊厥持续状态 胶质纤维酸性蛋白 白介素-1Β 细胞凋亡 依达拉奉 大鼠 

分 类 号：R965[医药卫生—药理学]