串联填充管式反应系统中高浓度乙醇连续发酵  

Very High Gravity Ethanol Continuous Fermentation in Reaction System Composed by Packing Tubular Reactors

作　　者：申渝[1,2] 白凤武[2] 

机构地区：[1]重庆工商大学废油资源化技术与装备教育部工程研究中心,重庆400067 [2]大连理工大学生命科学与技术学院,大连116023

出　　处：《中国生物工程杂志》2011年第3期66-70,共5页China Biotechnology

基　　金：国家"863"计划(2007AA10Z358);重庆市教委科研项目(KJ100707);废油资源化技术与装备教育部工程研究中心科研项目(FYKJ2009010)资助项目

摘　　要：在一套由搅拌罐和管式反应器串联而成的组合式反应系统中,利用酿酒酵母进行连续发酵生产高浓度乙醇。后续管式反应器内通过装填聚氨酯颗粒和木块对酵母细胞进行吸附固定化,在乙醇抑制造成细胞活性大幅降低的情况下,通过大幅提高细胞浓度保证发酵效率,在稀释速率0.02h-1和280g/L葡萄糖的条件下,系统的终点乙醇浓度为15.4%(v/v)。研究表明在一定稀释速率之下,应该通过增加反应器的级数来降低稀释速率,以达到提高终点乙醇浓度,如简单地降低进料速率则可能增加整个系统所受的乙醇抑制,对提高终点乙醇浓度效果不显著。A fermentation system composed of a stirred tank followed by four-stage packed tubular bioreactors in series was set up to evaluate very high gravity ethanol continuous fermentation using Saccharomyces cerevisiae.Polyurethane and wood chips were used for cells immobilization in tubular reactors,and ethanol productivity in later reactors were maintained at high level by high bilomass content even the viability and ethanol productivity of cells were inhibited by high ethanol content.The system ran at 0.02h-1 and fermented very high gravity medium containing 280 g/L glucose to produced 15.4 % v/v ethanol.It was also proved that dilution rat should reduce by new reactors added to higher final ethanol content under the lower dilution rat level.

关 键 词：乙醇 高浓度连续发酵 组合式反应系统 细胞固定化 

分 类 号：Q815[生物学—生物工程]