绵羊腺病毒287作为基因传递载体的应用前景  

The Application Prospects of Ovine Adenovirus287 Vector

作　　者：谷元兴[1] 王猛[1] 刘文倩[1] 张杰[1] 刘永生[1] 

机构地区：[1]中国农业科学院兰州兽医研究所,兰州730046

出　　处：《中国生物工程杂志》2011年第3期101-106,共6页China Biotechnology

基　　金：国家"转基因生物新品种培育"重大专项资助项目(2009ZX08007-006B)

摘　　要：绵羊腺病毒287(OAdV287)具有独特的基因组结构,全长29 576bp。基因组最大的特点是有很高的A/T含量(66.4%)和缺少典型的E1区,以及含有一段较长的冗余片段。已识别出其基因组中有3个外源基因插入区,理论上最多可容纳6.3kb的外源DNA。OAdV287的纤维和五邻体结构结构独特,衣壳蛋白上缺少可识别的整合蛋白结合域。OAdV287能感染一系列人和动物的细胞系,但是不能在非绵羊源细胞内成功复制。OAdV287载体可以避免人腺病毒的免疫干扰,具有很高的转染效率。目前,OAdV287已成为研究最热的动物源腺病毒载体之一。Ovine adenovirus 287 has a unique genome organization with a length of 29 576bp.The genome is characterized distinctively by a high A/T content（66.4%）,the lack of a typical El region,and a long redundant sequence.Three nonessential regions of the viral genome,where 6.3kb foreign genes can be inserted theoretically,have been identified.OAdV287 also has unique fiber and penton,where lacks the integrin-binding domain in penton or any other capsid protein.OAdV287 can infect a wide range of human and animal cell lines,but failing to replicate in non-ovine cell lines.In addition,OAdV287 vector can circumvent the pre-existing human adenoviruses immunity,and so have an efficient transfection.Currently,OAdV287 has become one of the research focuses among the animal adenoviruses vectors.

关 键 词：绵羊腺病毒 基因组结构 基因载体 应用 

分 类 号：Q782[生物学—分子生物学]