高氧对肠上皮细胞表达分泌片的影响  

Effect of hyperoxia on the expression of secretory component in human intestinal epithelial Caco-2 cells

作　　者：刘冬妍[1] 苗佳宁[1] 

机构地区：[1]中国医科大学附属盛京医院实验研究中心卫生部小儿先天畸形重点实验室,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2011年第4期362-366,共5页World Chinese Journal of Digestology

基　　金：国家自然基金资助项目;No.30871158;辽宁省教委课题基金资助项目;No.2008783~~

摘　　要：目的:研究高氧对肠上皮细胞分泌片(SC)表达的影响.方法:采用细胞计数和Giemsa染色方法检测不同氧浓度对细胞生长和细胞分裂能力的影响,采用免疫组织化学方法检测不同氧浓度对Caco-2表达SC的影响.结果:细胞计数显示400mL/L氧浓度利于细胞生长;600、900mL/L氧浓度导致细胞迅速死亡.不同氧浓度干预细胞3d细胞分裂指数有明显不同,分裂细胞百分数分别为正常氧浓度2.5;400mL/L氧浓度为3.3;600mL/L氧浓度为1.3;900mL/L氧浓度大部分细胞死亡.与正常氧浓度相比,氧浓度为400、600mL/L时SC表达增强,900mL/L氧浓度时SC表达明显减弱,甚为阴性,但600mL/L氧浓度SC表达较400mL/L氧浓度减弱.结论:适度的高氧促进细胞生长和促进肠上皮细胞表达SC,严重高氧则抑制肠上皮细胞SC表达及肠上皮细胞生长,肠上皮细胞SC表达增多有助于保护肠黏膜及平衡肠黏膜作用,阻滞细菌入侵肠道.AIM：To explore the effect of hyperoxia on the expression of secretory component（SC） in human intestinal epithelial Caco-2 cells.METHODS：The number of Caco-2 cells was counted with a hemacytometer,and cell division was determined by Giemsa staining.The changes in the expression levels of SC in Caco-2 cells were detected by immunocytochemistry.RESULTS：Caco-2 cells exhibited exponential growth in air containing 400 mL/L O2.Cell growth was partially inhibited when the proportion of oxygen in air was elevated to 600 mL/L,and completely inhibited when elevated to 900 mL/L.The division index of treated cells was 2.5 in air,3.3 in 400 mL/L O2,and 1.3 in 600 mL/L O2.Many cells died in 900 mL/L O2.Compared with cells incubated in air,the expression of SC was up-regulated in 400 mL/L and 600 mL/L O2.The ability of intestinal epithelial cells to express SC was limited in 900 mL/L O2.Compared with cells incubated in 400 mL/L O2,the expression of SC was down-regulated in 600 mL/L O2.CONCLUSION：Moderately high concentrations of oxygen promote cell growth and SC expression in Caco-2 cells,whereas extremely high concentrations of oxygen inhibit cell growth and SC expression.High levels of SC are benef icial to maintaining and balancing the intestinal mucosa and inhibiting bacterial invasion.

关 键 词：高氧 肠上皮细胞 分泌片 细胞生长 细胞分裂 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]