IL-17、MMP-3在类风湿关节炎患者滑膜液及血浆中的表达及意义  

The Expression and Clinical Significance of IL-17 and MMP-3 in Synovial Fluid and Plasma in Patients with Rheumatoid Arthritis

作　　者：卢洁[1] 魏国忠[1] 戴利亚[1] 

机构地区：[1]温州市第三人民医院,325000

出　　处：《放射免疫学杂志》2011年第2期205-207,共3页Journal of Radioimmanology

摘　　要：目的:分析类风湿关节炎(RA)患者滑膜液及血浆中白细胞介素-17(IL-17)、基质金属蛋白酶-3(MMP-3)的水平及其临床意义。方法:应用酶联免疫吸附法(ELISA)检测RA患者滑膜液及血浆中IL-17及MMP-3的水平,分析IL-17及MMP-3与临床指标的关系。结果:RA患者滑膜液IL-17及MMP-3水平明显高于对照组(P均<0.01);RA患者活动组血浆IL-17及MMP-3水平明显高于稳定组(P均<0.01),而后者又明显高于对照组(P均<0.01);RA患者滑膜液及血浆中MMP-3水平与CRP、DAS28评分呈正相关性(P均<0.05),与RF不存在相关关系(P>0.05);RA患者滑膜液及血浆中IL-17水平与MMP-3、CRP、RF及DAS28评分均呈正相关(P均<0.05)。结论:RA患者的滑膜液及血浆中存在异常增高的IL-17及MMP-3,且其水平与病情活动相关,血浆IL-17可作为RA病情活动的参考指标之一。Objective To analysis the levels of interleukin（IL）-17 and matrix metalloprotienase（MMP）-3 in synovial fluid and plasma in patients with rheumatoid arthritis（RA） and to investigate it＇s clinical significance.Methods The levels of IL-17 and MMP-3 in plasma and synovial fluid in RA patients were tested by ELISA,simultaneously,The relationships among IL-17,MMP-3 and other clinical indexes were analyzed.Results IL-17 and MMP-3 levels in synovial fluid in patients with RA were significantly higher than those in control groups（P0.01）,the levels of plasma IL-17 and MMP-3 in active RA patients were apparently higher than those in stable ones（P0.01）,meanwhile the same case occurred between stable and control groups（P0.01）,the levels of plasma and synovial fluid MMP-3 showed positive relationship with CRP,DAS28（P0.05） but none with RF（P0.05）,the plasma and synovial fluid levels of IL-17 also shown positive relationship with MMP-3,CRP,RF,DAS28（P0.05）.Conclusion IL-17 and MMP-3,which have abnormal elevations in synovial fluid and plasma from patients with RA,its levels were well correlated to the activity of RA and the elevated plasma IL-17 levels may be the useful markers for active RA.

关 键 词：类风湿关节炎(RA) 白细胞介素-17(IL-17) 基质金属蛋白酶-3(MMP-3) 酶联免疫吸附法 

分 类 号：R392.11[医药卫生—免疫学]