柞蚕丝素/壳聚糖共混膜的结构及细胞相容性  

Structure and Cytocompatibility of Antheraea Pernyi Silk Fibroin/Chitosan Blend Films

作　　者：闫书芹[1] 赵婷[1] 徐亚梅[1] 张强[1] 李明忠[1,2] 

机构地区：[1]苏州大学纺织与服装工程学院,江苏苏州215021 [2]苏州大学现代丝绸国家工程实验室,江苏苏州215123

出　　处：《高分子材料科学与工程》2011年第4期52-55,共4页Polymer Materials Science & Engineering

基　　金：国家973计划课题(2005CB623902);国家自然科学基金资助项目(30970714);江苏省高校自然科学重大基础研究项目(07KJA43010)

摘　　要：用碳化二亚胺(EDC)作为交联剂,流延法制备柞蚕丝素(ASF)/壳聚糖(CS)共混膜。用扫描电镜(SEM)、红外光谱(FT-IR)、热重分析(TG)和四甲基偶氮唑盐比色法(MTT)对膜的结构及细胞相容性进行了研究。结果显示,柞蚕丝素和壳聚糖具有较好的相容性和较强的相互作用,壳聚糖能阻碍共混膜内的丝素蛋白形成β-折叠构象。EDC能分别与柞蚕丝素及壳聚糖反应,从而对共混膜进行有效的交联,并使膜的热稳定性提高。与ASF膜或CS膜相比,共混比为80/20和40/60的ASF/CS共混膜更有利于细胞生长和增殖,作为一种新型的生物材料具有良好的研究和开发应用前景。The blend films of antheraea pernyi silk fibroin（ASF）/chitosan（CS） were prepared by casting ASF/CS mixture solution on a polyethylene plate and using carbodiimide hydrochloride（EDC） as crosslinking agent.Fourier transform infrared spectrum（FT-IR）,X-ray diffraction（XRD）,differential scanning calorimetry（DSC） and thermogravimetric analysis（TGA）were used to analyze the structure and cytocompatibility of the blend films.Results indicate that ASF and CS have good compatibility and strong interactions,CS interferes with ASF to form β-sheet structure.EDC can react with ASF and CS respectively and has obvious crosslinking effect on the blend films,the thermal stability of the blend films is also improved.Compared with ASF or CS films,the ASF/CS blend films with blend ratio 80/20 and 40/60 are more favorable for the growth and proliferation of cells.As novel biomaterials,the ASF/CS blend films have bright prospects on research and application.

关 键 词：生物材料 丝素 壳聚糖 结构 细胞相容性 

分 类 号：TB383[一般工业技术—材料科学与工程]