实时荧光定量PCR检测疫霉侵染下辣椒nsLTP基因的差异表达  

REAL-TIME FLURESCENCE QUANTITATIVE PCR FOR DECTECTING THE DIFFERENTIAL EXPRESSION OF THE NSLTP GENE IN PEPPER(CAPSICUM ANNUUM L.) UNDER PHYTOPHTHORA CAPSICI STRESS

作　　者：贺俐[1] 吴杨[1] 

机构地区：[1]井冈山大学生命科学学院,江西吉安343009

出　　处：《井冈山大学学报（自然科学版）》2011年第2期112-115,131,共5页Journal of Jinggangshan University (Natural Science)

基　　金：国家自然科学基金项目(31060263);江西省自然科学基金项目(2009GQN0077);江西省教育厅科技计划项目(GJJ11537);井冈山大学校级科研课题项目(JZ09016)

摘　　要：采用实时荧光定量PCR方法测定了辣椒疫霉菌（Phytophthora capsici.）胁迫下辣椒抗病基因nsLTP的转录水平变化。结果表明：与对照相比,疫霉胁迫下nsLTP基因表达量随着胁迫时间延长呈现出先升高后降低再升高的变化特点,该基因在早期被诱导表达,在胁迫6h后表达量达到最大值,之后明显下降,相对表达量变化范围为2.38~15.53。The differential expression of nsLTP gene in pepper under Phytophthora capsici Stress was analyzed by real-time fluorescence quantitative polymerase chain reaction.The results indicated that compared with control,nsLTP gene transcription level in pepper leaves follows a high-low-high pattern under Phytophthora capsici Stress,nsLTP gene inductively expressed in the early stage of stress,reached the peak 6 hours after treatment,and then decreased significantly.The relative expression ratios of the nsLTP gene ranged from 2.38 to 15.53.

关 键 词：辣椒 疫霉 非特异性脂转移蛋白 荧光定量PCR 

分 类 号：Q945.78[生物学—植物学] Q78