CpG ODN佐剂对呼吸道合胞病毒重组疫苗诱导的细胞免疫应答的作用  

Role of CpG ODN adjuvant in the cellular immune response induced by recombinant vaccine of respiratory syncytial virus

作　　者：李娜[1] 刘建勋[1] 曾瑞红[2] 

机构地区：[1]邢台医学高等专科学校,邢台054000 [2]河北医科大学免疫教研室,石家庄050017

出　　处：《中国免疫学杂志》2011年第4期291-294,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金(30972801);教育部科技重点项目(209009);高等学校博士学科点专项基金(20091323120005)资助项目

摘　　要：目的:研究CpG2216佐剂对呼吸道合胞病毒(RSV)重组蛋白疫苗诱导的细胞免疫应答的作用。方法:重组RSV疫苗G1F/M2与CpG2216佐剂混合,或与CpG2216及常规佐剂Al(OH)3混合,鼻腔(i.n.)或腹腔注射(i.p.)免疫BALB/c小鼠三次,最后一次免疫后2周杀小鼠,取脾细胞,用乳酸脱氢酶(LDH)释放法检测脾细胞特异性杀伤活性;用ELISPOT法检测分泌IFNγ-和IL-4的细胞;用流式细胞仪检测CD4+/CD8+效应及LDH记忆细胞。结果:与G1F/M2相比,CpG+G1F/M2鼻腔或腹腔注射免疫均诱导了显著的杀伤活性;而且G1F/M2+Al+CpG(i.p.)诱导的杀伤活性显著高于CpG+G1F/M2(i.p.)。ELISPOT结果显示:CpG+G1F/M2鼻腔免疫和腹腔注射免疫组的分泌IFNγ-和IL-4的淋巴细胞数量明显多于G1F/M2组;CpG+Al+G1F/M2(i.p.)组的细胞数显著多于CpG+G1F/M2(i.p.)组;且各组分泌IFNγ-的淋巴细胞数显著多于分泌IL-4的淋巴细胞,即均诱导了Th1型优势应答,有利于宿主抗病毒。流式细胞仪检测结果表明:CpG+G1F/M2(i.n.)仅诱导CD44+单阳性的细胞,而CpG+G1F/M2(i.p.)和CpG+Al+G1F/M2(i.p.)既诱导产生了CD44+单阳性细胞,也产生了CD44+CD62L+双阳性的记忆细胞。结论:CpG2216作为RSV重组疫苗G1F/M2的佐剂,可显著增强细胞免疫应答。Objective：To investigate the role of CpG ODN adjuvant in the cellular immune responses induced by recombinant protein vaccine of respiratory syncytial virus（RSV）.Methods：BALB/c mice were immunized i.n.or i.p.with G1F/M2＋CpG or G1F/M2＋Al＋CpG three times.Spleens from the immunized mice were removed two weeks after the third immunization.Specific lytic activity was assessed using a LDH Cytotoxicity Assay Kit.IFN-γ-and IL-4-secreting cells were quantified using a ELISPOT kit.Percentages of CD4＋ /CD8＋ effector cells or LDH memory cells in the splenic cells of mice were analyzed with flow cytometer.Results：Immunization with G1F/M2＋CpG i.n.or i.p.induced significant specific lytic activity compared to G1F/M2.The specific lytic activity induced by G1F/M2＋Al＋CpG（i.p.） was significantly higher than that by G1F/M2＋CpG（i.p.）.G1F/M2＋CpG i.n.or i.p.induced significant more IFN-γ-secreting cells and IL-4-secreting cells than G1F/M2 i.n.or i.p.significantly.The level of IFN-γ-secreting cells or IL-4-secreting cells in G1F/M2＋Al＋CpG（i.p.） group was higher than that in G1F/M2＋CpG（i.p.） group.In addition,the level of IFN-γ-secreting cells was remarkably higher than that of IL-4-secreting cells in each group,suggesting a Th1 biased response,which benefited for defensing virus.G1F/M2＋CpG（i.n.） induced only CD44＋CD62L-cells.G1F/M2＋Al＋CpG（i.p.） or G1F/M2＋CpG（i.p.） elicited both CD44＋CD62L-cells and CD44＋CD62L＋ cells.Conclusion：CpG2216,as an adjuvant,can enhance significantly the cellular immune responses of RSV recombinant vaccine G1F/M2.

关 键 词：CpG2216 佐剂 呼吸道合胞病毒(RSV) 重组蛋白疫苗 细胞免疫应答 

分 类 号：R373.21[医药卫生—病原生物学]