信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨  

The roles of IRAK1/4 in anti-β2GPI/β2GPI-induced tissue factor expression in THP-1 cells

作　　者：文海平[1] 周红[1] 许国莹[1] 郭东琳[1] 周芳[1] 陈东东[1] 解鸿翔[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,镇江212013

出　　处：《中国免疫学杂志》2011年第4期295-299,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.30670907;30971301);江苏大学学生科研课题项目(09A081)资助

摘　　要：目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。Objective：To investigate the role of IRAK1 and IRAK4 in tissue factor expression of THP-1 cells treated with anti-β2GPI/β2GPI complex.Methods：The TF expression of THP-1 cells was detected by Real-time PCR or TF activity kits after stimulating with anti-β2GPI/β2GPI complex.The IRAK1,p-IRAK1 and IRAK4 expression induced by anti-β2GPI/β2GPI complex was determined by Western blot.It was also investigated that IRAK1/4 inhibitor could interrupt the TF expression in THP-1 cells stimulated with anti-β2GPI/β2GPI complex.Results：The TF expression（both mRNA and activity） of THP-1 cells could be significantly up-regulated with the treatment of anti-β2GPI/β2GPI complex（100 μg/ml）.The IRAK1,p-IRAK1 and IRAK4 expression（protein） in THP-1 cells was significantly increased by stimulation of anti-β2GPI/β2GPI complex（100 μg/ml）.IRAK1/4 inhibitor（50 μmol/L） could decrease the levels of TF and p-IRAK1 in THP-1 cells induced by anti-β2GPI/β2GPI complex.Conclusion：IRAK1 and IRAK4 can be activated and contributed to TF expression of THP-1 cells induced with anti-β2GPI/β2GPI complex.

关 键 词：抗磷脂综合征 antiβ-2GPI/β2GPI IRAK1 IRAK4 组织因子 

分 类 号：R593.2[医药卫生—内科学]