红藤对LPS诱导流产小鼠的保胎作用及子宫巨噬细胞的影响  

Effects of sargentodox cuneata on tocolysis role and the macrophages in the uteri of abortion mice induced by LPS

作　　者：王卫华[1] 闫坤[1] 王丽霞[1] 吴雷振[1] 张瑞平[1] 张顺利[1] 

机构地区：[1]河南师范大学生命科学学院省部共建细胞分化调控国家重点实验室,新乡453007

出　　处：《中国免疫学杂志》2011年第4期325-329,共5页Chinese Journal of Immunology

基　　金：河南省科技攻关项目(No.0524420040);河南基础与前沿技术项目(No.092300410193)资助

摘　　要：目的:探讨红藤对细菌脂多糖(LPS)诱导流产小鼠的保护作用及其免疫药理机制。方法:昆明种小鼠55只,随机分为对照组(A组,10只)和实验组。实验组又分为LPS处理组(B组)、红藤处理组(C组)、红藤及LPS双处理组(D组),每组各15只。观察妊娠结果,并用酶组织化学、免疫组化、ELISA方法检测子宫非特异性酯酶阳性(α-NAE+)、CD14+、CD204+巨噬细胞和TNF-α的变化。结果:小鼠流产率在B组为100%(P<0.01),胚胎吸收率为100%(P<0.01);而D组流产率降低为13.33%,胚胎吸收率降至12.56%;孕鼠子宫α-NAE+、CD14+巨噬细胞数量,B组子宫各部位与A组相比均显著增多(P<0.01);D组环肌外侧与A组相比显著增多(P<0.01),但环肌内侧、功能层无明显变化,C、D组CD204+巨噬细胞数量与A组、B组相比均显著增多(P<0.01);子宫TNF-α含量B组与A组相比显著增加(P<0.01),而D组接近正常水平。结论:红藤可以对抗LPS所致的小鼠流产,通过影响孕鼠子宫巨噬细胞的数量、分布和亚群,抑制TNF-α的分泌,是其保护胚胎正常发育的机理之一。Objective：To explore the immunosuppression effects and tocolysis of sargentodox on abortion mice induced by LPS.Methods：Mice of Kunming（55 mice） were divided into control group（group A,10 mice） and experimental group.Experimental group were divided into group B（intraperitoneal injection LPS）;group C（ingestion sargentodox）,and group D（ingestion sargentodox and intraperitoneal injection LPS）,each group of 15 mice.Then the pregnancy results were obversed,and the varity of α-NAE＋,CD14＋,CD204＋macrophages and TNF-α in the uteri were identified by enzyme-histochemistry,immunohistochemistry and ELISA.Results：The abortion rate of group B is 100%（P〈0.01）,The embryo resorbing rate is 100%（P〈0.01）,but there was the decreased abortion rate of 13.30% in group D.The embryo resorbing rate decreased to 12.56%.The number of α-NAE＋ and CD14＋macrophages in the uteri of gestation mice of group B was greatly increased comparing with group A（P〈0.01）.These effects outside of myometrium of group D were remarkably increased comparing with group A（P〈0.01）.but there was no distinct difference in the inside of myometrium and function layer.The number of CD204＋macrophages in group C and D was greatly increased comparing with group A and B（P〈0.01）.The TNFα contents in the uteri of mice in group B were greatly increased comparing with group A（P〈0.01）,but close to normal levels in group D.Conclusion：The effect of miscarriage induced by LPS can be antagonized by sargentodox,through decreasing the quantity,distribution and sub-groups of macrophages in the uteri of gravidity mice.Inhibited secretion of TNF-α is one of the mechanisms for maintaining normal embryonate development.

关 键 词：红藤 巨噬细胞 CD14 CD204 TNF-Α 流产 小鼠 

分 类 号：R967[医药卫生—药理学]