新疆细粒棘球蚴免疫诊断抗原B亚单位3基因的克隆及序列分析  

Cloning and sequence analysis of potential diagnosis gene of Echinococcus granlosus in Xinjiang

作　　者：马海梅[1,2] 陈洁[3,2] 古力帕丽.麦曼提依明 陈璐[3,2] 丁剑冰[1,2] 吾拉木.马木提 

机构地区：[1]新疆医科大学基础医学院病原学教研室,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院包虫病重点实验室,新疆乌鲁木齐830011 [3]新疆医科大学基础医学院寄生虫教研室,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2011年第3期236-239,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(30760229);科技部"863计划项目"(2007AA02Z411);新疆维吾尔自治区高校科研计划(XJEDU2008S31)

摘　　要：目的确定细粒棘球蚴抗原B亚单位3(EgAgB8/3)的特性并为研究其免疫功能奠定基础。方法根据已报道的编码EgAgB8/3的基因序列(AF362442)设计引物,进行基因扩增,利用DNAstarProtean软件对该抗原进行分析。结果成功克隆到EgAgB8/3基因,扩增片段为207 bp;序列比对结果显示,新疆细粒棘球蚴EgAgB8/3基因与GenBank登录号为AF362442序列完全一致,同源性为100%。对该抗原分析认为具有潜在的抗原表位位点。结论细粒棘球蚴EgAgB8/3在对包虫病的免疫诊断上是较好的候选抗原,并为研究该蛋白的免疫功能及建立以EgAgB8/3抗原为主的包虫病终末宿主免疫诊断方法奠定了基础。Objective To further study the character of Echinococcus granulosus antigen B protein subunit B8/3（EgAgB8/3） gene and provide the basis for analysis of its immunological function.Methods The specific primers were designed according to published nucleotide sequence of Echinococcs granulosus EgAgB8/3 gene in the Genbank database（AF362442）.The fragment of EgAgB8/3 gene was amplified by PCR and analyzed by DNA star software.Results The EgAgB8/3 gene was successfully cloned and the full length of the cloned gene was 207 bp.The EgAgB8/3 gene showed 100% homology with the foreign strain（AF362442）.Several possible antigen epitopes located in the EgAgB8/3.Conclusion EgAgB8/3 has the potential immunological diagnosis value,it provides the basis for further establishing the immunological diagnosis method of Eg.

关 键 词：细粒棘球蚴 抗原B8/3 聚合酶链反应(PCR) 

分 类 号：R383.3[医药卫生—医学寄生虫学]