含多种抗真菌病基因植物表达载体的构建  

The Construction of Plant Expression Vector Containing Multi Antifungal Genes

作　　者：李明[1] 邱国华[1] 许新萍[1] 许耀[1] 李宝健[1] 

机构地区：[1]中山大学生物工程研究中心,广州510275

出　　处：《中山大学学报（自然科学版）》1999年第5期67-71,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家863 高技术计划

摘　　要：含有大麦核糖体失活蛋白（ＢＲＩＰ） 编码序列的质粒ｐＲＣ２４／ＢＲＩＰ改造后， 依次与含有水稻碱性几丁质酶基因ＲＣＨ１０ 和水稻酸性几丁质酶基因ＲＡＣ２２ 编码序列的质粒ｐＡＢＣ２ 以及含有苜蓿β１ ， ３葡聚糖酶编码序列的质粒ｐＡＡＧ８９ 连接， 得到了中间载体ｐＲＡＳ１０ ．ｐＲＡＳ１０再与根癌农杆菌双元载体ｐＣＡＭＢＩＡ１３００ 连接， 得到了适合水稻转化的双元载体ｐＲＡＳ１３００ ． 利用冻融法将此表达载体导入根癌农杆菌ＬＢＡ４４０４ 中， 并准备将ｐＲＡＳ１３００ 转入水稻，Mediate vector pRAS10 was obtained by ligating the modified plasmid pRC24/B RIP containing barley\|ribosome inactivating protein(B RIP) coding sequence with plasmid pABC2 containing rice basic chitinase gene RCH10 and rice acidic chitinase gene RAC22 coding sequence and then with plasmid pAAG89 containing alfalfa β 1,3 glucanase coding sequence pRAS10 was ligated to Agrobacterium tumefaciens binary vector to produce plant expression vector pRAS1300 which was suitable for rice transformation The expression vector was transformed into A tumefaciens LBA4404 using the Freeze shaw method The rice transformation using the constructed plant expression vector is in progress, in order to obtain rice varieties having broad and strong anti fungi traits.

关 键 词：真菌病 植物表达载体 构建 抗真菌病基因 

分 类 号：S332.2[农业科学—作物遗传育种] S432[农业科学—农艺学]