染料脱色酶特性研究  

STUDY ON CHARACTERIZATION OF DECOLORIZING ENZYME

作　　者：宋文华[1] 颜慧[1] 冯炘[1] 李军红[1] 胡国臣[1] 温孚江[1] 戴树桂[1] 

机构地区：[1]南开大学环境科学与工程学院,天津300071

出　　处：《南开大学学报（自然科学版）》1999年第3期146-150,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金;天津市自然科学基金

摘　　要：从脱色优势菌ＮＤ１ 提取脱色酶，对酶特性进行研究．结果表明酶的最适作用条件为ｐＨ ７，３７℃，无氧环境，反应需ＮＡＤＨ 的参与；纯化结果表明经ＤＥＡＥ柱层析及Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析后脱色速率分别提高１０ 倍和５０倍，Ｓｅｐｈａｄｅｘ Ｇ－１００ 凝胶层析中２～４ 管蛋白具有偶氮和蒽醌染料脱色酶活性，６～１２ 管具有蒽醌染料脱色酶活性．In this paper, decolorizing enzyme of superior strain ND1 was extracted and purified, and the characterization of enzyme was studied. The result showed that the optimum condition was pH7.0, 37℃,anaerobic, NADH added. By DEAE and Sephadex G 100 chromatogram purified, the decoloriziing rate of the enzyme was improved 10 and 50 times respectively, and in Sephadex G 100 chromatogram, the protein in tube 2～4 was in charge of the decolorization of zao and anthraquinone dyes, tube 6～12 was in charg of decolorizing enzyme activity of anthraquinone dye.

关 键 词：脱色酶 纯化 染料 废水处理 微生物降解 

分 类 号：X791.03[环境科学与工程—环境工程]