JD病毒基因组片段的克隆和序列分析  

CLONING AND SEQUENCING OF GENOMIC FRAGMENTS FROM JEMBRANA DISEASE VIRUS

作　　者：王金忠 梁栋[2] 刘淑红[2] 陈启民[2] 赵祥平[2] 侯艳梅[2] 耿运琪[2] 

机构地区：[1]天津市动植物检疫局,天津300457 [2]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》1999年第3期151-154,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：天津市自然科学基金

摘　　要：扩增并克隆了ＪＤ病毒的ｇａｇ 基因片段（位于３００ｎｔ～５６４ｎｔ，编码基质蛋白ＭＡ）和ｐｏｌ基因片段（位于３４６７ｎｔ～３６９１ｎｔ，编码反转录酶ＲＴ），测定其序列并同ＢＩＶ、ＢＦＶ 和ＢＬＶ 的相应基因进行比较．所建立的方法能够特异性扩增ＪＤＶ序列。Two fragments from Jembrana disease virus (JDV) genome were amplified by PCR. The first located in 300nt to 564nt region of gag gene, encoding viral matrix protein (MA). The second located in 3467nt to 3691nt region of pol gene, encoding the reverse transcriptase (RT). The fragments were cloned into pUC18 plasmid and confirmed by DNA sequencing, then were compared with gag and pol genes of bovine immunodeficiency virus (BIV), bovine foamy virus (BFV), and bovine leukemia virus (BLV). It can be used to detect the JDV provirus by PCR analysis as described.

关 键 词：JD病毒 序列分析 基因组 克隆 牛病毒 慢病毒 

分 类 号：S852.653[农业科学—基础兽医学]