IM-94增强小鼠免疫功能的实验研究  

IM 94 IMPROVES THE IMMUNOLOGIC FUNCTION OF MICE

作　　者：顾海明[1] 卢是月[2] 刘宇峰[1] 徐力致[1] 廖红[1] 张祖暄[1] 

机构地区：[1]南京大学医学院,南京210093 [2]中国人民解放军海军医学高等专科学校,南京210099

出　　处：《南京大学学报（自然科学版）》1999年第5期515-519,共5页Journal of Nanjing University（Natural Science）

摘　　要：研究了ＩＭ９４ 对免疫低下小鼠免疫功能的影响．结果显示：ＩＭ９４ 能逆转环磷酰胺所致的免疫低下反应，并进一步提高小鼠的免疫活性，使其巨噬细胞吞噬能力、ＮＫ 细胞自然杀伤活性、淋巴细胞增殖水平、半数溶血值和抗体生成细胞水平均有明显提高，说明ＩＭ９４ 对实验小鼠有正向免疫调节作用．IM 94 is an active antitumor component,which is screened and purified from animal secretion according to traditional Chinese medical science and modern scientific knowledge.Primary active elements of IM 94 have been defined as 2 binding proteins with molecular weights of 17kD and 20kD, respectively.In the present paper,the effects of IM 94 on the immunologic function of mice were investigated by immunological techniques and biochemical analysis.It was found that higher doses of IM 94 could not only reverse cyclophosphamide induced low immunologic function,restoring it to the control level,but also raise immunologic response to a higher level well above the normal.The detailed results were as follows:1)25mg/kg,50mg/kg and 100mg/kg IM 94 could all obviously improve phagocytic index and no significantly different effect was found among the 3 doses;2)All the 3 doses of IM 94 administrated in the test(25mg/kg,50mg/kg and 100mg/kg)could greatly increase the number of plaques in a dose dependent manner;3)Higher doses of IM 94(50mg/kg～100mg/kg or 200mg/kg)could enhance lymphocyte transformation ability,increase value of HC 50 ,microphage phagocytic function and improve NK cell activity,while 25mg/kg IM 94 had few effects on those functions. Past and present findings indicate that IM 94 can be considered as a promising immunopotentiator as well as an antitumor agent with little side effect and good efficacy.

关 键 词：IM-94 免疫功能 环磷酰胺 肿瘤细胞 抑制作用 

分 类 号：R392[医药卫生—免疫学] R967[医药卫生—基础医学]