Fe^(2+)对雄性泥鳅生殖毒性的研究  

Research about Reproductive Toxicity of Fe^(2+) to the Male Loach,Misgurnus anguillicaudatus

作　　者：陈玉明[1] 陈杰[1] 王曰文[1] 胡家会[1] 陈凤梅[1] 

机构地区：[1]聊城大学生命科学学院,山东聊城252059

出　　处：《湖北农业科学》2011年第7期1444-1447,共4页Hubei Agricultural Sciences

基　　金：山东省自然科学基金项目(ZR2010CM048)

摘　　要：以泥鳅(Misgurnus anguillicaudatus)精子为受试材料,应用计算机辅助精子分析(CASA)系统,研究了亚铁离子(Fe2+)对雄性泥鳅的生殖毒性。在预实验的基础上,设置30、60和300μmol/L 3个Fe2+浓度梯度。泥鳅精子暴露0、0.5、1.0、2.0和4.0h后激活,测定运动百分数、时间和速度等精子运动参数。结果表明,60mol/L的Fe2+溶液明显影响泥鳅的精子运动时间和相对速度(P<0.05)。高浓度的Fe2+能够抑制泥鳅精子运动,对泥鳅具有潜在生殖毒性作用。Reproductive toxicity of Fe2＋ on the sperm of loach（Misgurnus anguillicaudatus） was examined by a Computer-Assisted Semen Analyzer（CASA）.Based on the preliminary experiment,the loach sperm was incubated in preservation solutions containing Fe2＋（30,60,and 300 μmol/L） for 0,0.5,1.0,2.0 and 4.0 h,respectively.The percentage,duration,and velocity of sperm motility were determined in activator containing the same concentration of iron ion of preservation solutions.The results showed that the duration and relative velocity reduced significantly at 60 mol/L of Fe2＋（P0.05）.The results suggested that high concentration of ferrous ions would significantly damage the sperm motility of the loach,and might have harmful effect on the loach reproduction.

关 键 词：亚铁离子(Fe2+) 泥鳅 精子运动 生殖毒性 

分 类 号：R994.6[医药卫生—毒理学]