拟南芥抗日本曲霉毒素SAF突变体的筛选  

Screening of Arabidopsis thaliana Resistant Mutants against Aspergillus japonicus Toxin SAF Using Physiological and Biochemical Methods

作　　者：李明[1] 曾任森[2] 骆世明[2] 

机构地区：[1]广东药学院中药学院,广州510006 [2]华南农业大学热带亚热带植物生态研究所,广州510642

出　　处：《湖北农业科学》2011年第7期1480-1483,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30570283);广东省自然科学基金博士启动基金项目(05300830);广东药学院科研启动基金项目(43548017)广东药学院中青年骨干教师专项基金项目(2008)

摘　　要：黑麦酮酸F(SAF)是从日本曲霉(Aspergillus japonicus)中分离的化感物质。以拟南芥为材料,在萌发期和幼苗期分别以0.250、0.300 mmol/L SAF为选择浓度,根据种子是否萌发和幼苗能否存活为指标,并结合细胞死亡、MDA含量、H2O2含量等植物生理生化指标初步筛选得到拟南芥抗SAF突变体Hst 1,为进一步研究SAF的化感作用机理提供试验材料,也为拟南芥突变体的快速筛选技术提供参考依据。Secalonic acid F（SAF） was an allelochemical produced by Aspergillus japonicus.Arabidopsis as material,0.25 mmol/L and 0.3 mmol/L SAF were used as the selection concentrations at germination and seedling stage respectively.According to the seed germination percentage and seedling survival rate,combined with the physiological and biochemical indexes,such as cell death rate,MDA content,H2O2 content,Arabidopsis mutants Hst 1 resistant to SAF was screened primarily.This investigation would provide experimental materials to the further study on the mechanism of allelopathy of SAF,and provide some references for the rapid screening technology of Arabidopsis mutants.

关 键 词：拟南芥 黑麦酮酸F 突变体 

分 类 号：S335[农业科学—作物遗传育种] Q949.748.3[农业科学—农艺学]