保护剂减轻氯嘧磺隆对玉米残留药害的作用机理  

Mechanism of Herbicide Safener for Reducing the Phytotoxicity of Chlorimuron-ethyl to Maize

作　　者：郭玉莲[1,2,3] 

机构地区：[1]黑龙江省农业科学院博士后工作站,哈尔滨150086 [2]东北林业大学博士后流动站,哈尔滨150040 [3]黑龙江省农科院农药应用研究中心,哈尔滨150086

出　　处：《农业环境科学学报》2011年第4期645-649,共5页Journal of Agro-Environment Science

基　　金：黑龙江省农科院博士后基金项目(LRB08-350);哈尔滨市科技攻关项目(2008AA6CH047)

摘　　要：采用沙培方法,利用生理生化试验及半定量PCR方法,研究了保护剂HN对玉米ALS、GSTs活性及GSTs的mRNA表达量的影响。结果表明,保护剂对6个玉米品种的ALS、GSTs均具有诱导作用,但对不同品种的诱导增加百分率却不同。对氯嘧磺隆敏感的玉米东甜3号的GSTs诱导增加百分率达到59.18,对其ALS的诱导倍数为叶部1.29倍,根部2.30倍;对氯嘧磺隆耐性较高的玉米屯玉88GSTs诱导增加百分率为19.26,其ALS的诱导倍数为叶部1.07倍,根部2.07倍。氯嘧磺隆和保护剂均可以诱导玉米GSTsmRNA的相对表达量的增加,保护剂的诱导作用更强。Herbicide safener can reduce phytotoxicity of herbicides to crops by influencing absorption and conduction of crops,and inducing the activity increase of GSTs and its target enzyme ALS.There may be two mechanisms of inducing the increase of GSTs activity.One is to induce the original GSTs isoenzymes over-expressed,the other is to induce synthesis of new GSTs isoenzymes.In this paper,physiological and biochemical tests and semi-quantitative PCR method were employed to study the effect of herbicide safener HN on the activity ALS and GSTs of maize as well as the effect of both of chlorimuron-ethyl and safener HN on the GSTs mRNA expression.The results showed that herbicide safener HN had effect of induction on ALS and GSTs of six maize varieties,but the increase percentage on different varieties showed a little difference.For chlorimuron-ethyl sensitive maize variety Dongtian 3,the inducing percentage of GSTs reached 59.18%,while its ALS increased 1.29 times in leaf and 2.30 times in root.For chlorimuron-ethyl tolerant maize variety Tunyu 88,the inducing percentage of GSTs was 19.26%,while its ALS increased 1.07 times in leaf and 2.07 times in root.Both of chlorimuron-ethyl and herbicide safener could induce relative increasing expression of GSTs mRNA and herbicide safener had stronger induction than chlorimuron-ethyl.

关 键 词：保护剂 氯嘧磺隆 玉米 GSTS MRNA表达量 

分 类 号：X592[环境科学与工程—环境工程]