西南地区水稻细菌性条斑病菌的遗传多样性分析  

Genotypic Diversity Analysis of Xanthomonas oryzae pv.oryzicola in Southwest China by Rep-PCR

作　　者：林琳[1,2] 姬广海[2] 马改转[2] 王永吉[2] 张丽辉[2] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学教育部农业生物多样性与病虫害控制重点实验室,云南昆明650201

出　　处：《江西农业大学学报》2011年第2期264-269,共6页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(30760139);农业部公益性行业科研专项(3-23)

摘　　要：选取云南、贵州、四川不同省市地区的水稻条斑病菌菌株141株,利用Rep-PCR技术筛选出多态性较好的引物对其DNA进行多态性分析。引物J3、ERIC、JELIR的扩增多态性较好,不同毒性菌株间的Rep-PCR指纹图谱差异很大。通过聚类分析显示,我国西南地区的水稻细菌性条斑病菌群体遗传分化明显,菌株的遗传族群与其致病性具有一定的相关性,强、中、弱毒性菌株大致归为不同的类群。因此,Rep-PCR技术可用于检测水稻细菌性条斑病菌的遗传变异与鉴定研究。The genetic diversity of 141 strains of Xanthomonas oryzae pv.oryzicola from Yunnan,Guizhou and Sichuan provinces was analyzed polymorphously by Rep-PCR.Primer J3,ERIC and JELIR had better amplified polymorphism,different toxicity strains of the Rep-PCR fingerprints varied widely.Cluster analysis showed that significant genetic diversity exists within the population of Xanthomonas oryzae pv.oryzicola in Southwest China.There was a high degree of correlation between the genetic strains populations of and their pathogenicity.Strains of strong,middle or weak toxicity were roughly classified into different genetic populations.The result suggests that Rep-PCR technique can be used for detecting genetic variation among strains of Xanthomonas oryzae pv.oryzicola and identification of strains.

关 键 词：水稻细菌性条斑病菌 小种 遗传多样性 REP-PCR 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]