检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
机构地区:[1]武汉生物工程学院生物工程系,湖北武汉430415
出 处:《江西农业大学学报》2011年第2期360-363,374,共5页Acta Agriculturae Universitatis Jiangxiensis
基 金:武汉市高校科学研究资助项目(2008K079);湖北省教育厅科学技术研究项目(B20094008)
摘 要:为探索银杏液体悬浮细胞培养条件下产生黄酮的工艺条件,以银杏液体悬浮系为试验材料,在250 mL的三角瓶中装入80 mL液体培养基,置于25℃,100 r/min转速下摇床培养,试验设计分别为光照与黑暗、培养时间、细胞聚集体的大小、前体物对银杏液体悬浮细胞系产生黄酮的影响。结果表明:光照条件下培养14 d黄酮含量最高,3~4 mm粒径的细胞聚集体是黄酮合成的适宜细胞聚集体,前体物L-苯丙氨酸和乙酸钠对黄酮合成无明显的促进作用,且对细胞生长有抑制作用。结论:初步探索到银杏液体悬浮细胞培养条件下产生黄酮的工艺条件,为银杏细胞悬浮培养工业化生产黄酮类物质提供一定的理论基础和技术参数。Objective:Conditions for producing flavonoids were studied by the cell suspension culture of ginkgo.Methods: In cell suspension cultures,the suspension was placed in 80 mL of liquid medium in a 250 ml-flask and kept on reciprocal shaker(100 r/min) at 25 ℃.The conditions of suspension cultures detected were as follows: light/darkness,culture time,size of cell aggregates and content of precursors.Results: The production of flavonoid reached a maximum by culture for 14 days under light.The cell aggregates with 3~4 mm of diameter were suitable for flavonoids synthesis.L-phenylalanine and sodium acetate as precursors had no significant promotion on flavonoids synthesis,however the cell growth was inhibited.Conclusion: Conditions for producing flavonoids were obtained by the cell suspension culture of ginkgo,which will further optimize the culture conditions required for the industrial production of flavonoids by the cell suspension culture of ginkgo.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.249