苯甲酰芍药甙对冠心病模型大鼠心肌细胞凋亡的影响  

Effects of Benzoylpaeoniflorin on apoptosis of Rats with Coronary Heart Diseases

作　　者：张二力[1] 李洋[2] 戈宏焱[1] 洪英杰[1] 董宇翔[1] 许丹[1] 李有田[1] 华树成[1] 

机构地区：[1]吉林大学第一医院中医科,吉林长春130021 [2]北京大学医学部基础医学院,北京100083

出　　处：《中国实验诊断学》2011年第4期609-610,共2页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省科技厅资助课题(20030903)

摘　　要：目的探讨苯甲酰芍药甙对冠心病模型大鼠心肌细胞凋亡的影响。方法高脂饲料饮食制备冠心病大鼠动物模型,给予苯甲酰芍药甙灌胃治疗4周,采用免疫组化方法检测心肌组织中Bc1-2、Bax的蛋白表达。结果同正常组比较,模型组大鼠Bc1-2显著降低而Bax表达增高(P<0.05);治疗后,治疗组大鼠Bcl-2表达增高、Bax表达降低,与模型组对比差异显著(P<0.05),同正常组比较无显著差异性(P>0.05)。结论苯甲酰芍药甙可显著提高冠心病大鼠Bcl-2蛋白的表达(P<0.05)、抑制Bax蛋白表达、增加Bcl-2/Bax比值,从而抑制心肌细胞凋亡。提示苯甲酰芍药甙可能通过抑制心肌细胞的凋亡而治疗冠心病。Objective To Study the effects of benzoylpaeoniflorin on apoptosis of rats with coronary heart diseases.Methods Experimental rats model with coronary heart diseases caused by high fat foods were prepared and benzoylpaeoniflorin were given for 4 weeks.Bc1-2、Bax in cardiac muscles of rats were detected with immunohistochemistry.Results Compared with that in normal group,Bc1-2 is lower and Bax is higher in model group（P0.05）;After treatment,Bc1-2 increased and Bax decreased in treatment group.There is significant difference about Bc1-2 and Bax in treatment group and in model group（P0.05）.But there is no great difference between treatment group and normal group（P0.05）.Conclusion Benzoylpaeoniflorin can restrain apoptosis of rats with coronary heart diseases by increasing the levels of Bc1-2 and decreasing the levels of Bax,suggesting that benzoylpaeoniflorin may treat coronary heart disease by decreasing apoptosis.

关 键 词：苯甲酰芍药甙 冠心病 细胞凋亡 BAX BCL-2 

分 类 号：R285.5[医药卫生—中药学]