YD383和YD439对IL-4诱导的BJAB细胞CD23表达的影响  

Effect of YD383 and YD439 on the Expression of CD23 Induced by IL-4 in BJAB Cells

作　　者：笪宇蓉[1] 邢军[2] 董学易[3] 宋春华[4] 段宏泉[3] 杨洁[1] 

机构地区：[1]天津医科大学免疫教研室,300070 [2]天津医科大学口腔医院 [3]天津医科大学基础医学研究中心 [4]天津医科大学卫生统计学教研室

出　　处：《天津医药》2011年第3期243-246,共4页Tianjin Medical Journal

基　　金：国家863计划项目(项目编号:2007AA02Z115);天津市科技支撑计划国际合作项目(项目编号:08ZCGHHZ01900);天津市高等学校科技发展基金计划项目(项目编号:20070207)

摘　　要：目的:初步探讨2种化合物YD383、YD439对JAK/STAT6信号传导通路的抑制机制。方法:将不同剂量的化合物YD383和YD439加入对数生长期的BJAB细胞作用30min,终浓度为20μg/L的白细胞介素(IL)-4共同孵育3h后,收集细胞并加入PE标记的鼠抗人CD23抗体,流式细胞仪检测各细胞群体CD23分子的表达情况。结果:化合物YD383和YD439均能降低由IL-4诱导的BJAB细胞CD23分子的表达,且随着化合物剂量的增大,CD23分子的表达水平逐渐降低。2种化合物各自不同的剂量组间CD23分子表达的差异均有统计学意义(P<0.05)。结论:化合物YD383和YD439对IL-4诱导的CD23分子的表达均有明显的抑制作用,并且具有剂量依赖性。Objective： To examine the effect of compounds,YD383 and YD439,on the expression of CD23 induced by IL-4 in BJAB cells. Methods： The BJAB cells of exponential growing were treated with YD383 or YD439 in different concentrations for 30 minutes. Then cells were incubated with IL-4 （20 μg/L） for 3 h. Cells were collected and mixed with PE-labeled CD23 antibody. The expression of CD23 was detected by flow cytometry in different conditions. Results： The expression of CD23 induced by IL-4 in BJAB cells was significantly inhibited by YD383 and YD439 in a concentration dependent manner（P〈0.05）.There were significant differences in the expression of CD23 between different concentration groups of two kinds of compounds（P〈0.05）. Conclusion： YD383 and YD439 can inhibit the expression of CD23 induced by IL-4 in BJAB cells. The expression of CD23 decreased in the concentration-dependent manner.

关 键 词：STAT转录因子类 蛋白酪氨酸激酶类 信号传导 白细胞介素4 受体 IgE流式细胞术 细胞系 肿瘤 

分 类 号：R392.11[医药卫生—免疫学]