车前子多糖对糖尿病小鼠氧化应激的影响  

Effect of Plantain Seed Polysaccharide on Oxidative Stress in Diabetic Mice

作　　者：张然[1,2] 袁从英[3] 冯娜[1] 王素敏[1] 

机构地区：[1]河北医科大学基础医学院药理教研室,050017 [2]河北省沧州医学高等专科学校 [3]石家庄职业技术学院

出　　处：《天津医药》2011年第3期253-255,共3页Tianjin Medical Journal

基　　金：河北省自然科学基金资助项目(项目编号:C2007000822);河北省重点科研项目(项目编号:20090314)

摘　　要：目的:研究车前子多糖(PSP)对实验性四氧嘧啶糖尿病小鼠氧化应激的影响。方法:60只昆明种小鼠随机抽取8只为对照组,其余动物于造模前饥饿24h,采用一次性腹腔注射四氧嘧啶,诱发形成实验性四氧嘧啶糖尿病小鼠动物模型,共有32只小鼠造模成功。随机分为模型组和各PSP给药组(P1、P2、P3组),每组8只。比色法测定丙二醛(MDA)、一氧化氮(NO)水平和一氧化氮合酶(NOS)活性及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-PX)活性。观察PSP对上述各生化指标的影响。结果:与模型组比较,除了P1组血清、心脏SOD,血液CAT,心脏GSH-PX、肾脏NO和血清的NOS差异无统计学意义,小鼠血清和心脏的MDA水平均显著降低外,其他指标活性均显著升高。结论:PSP具有抗氧化效应及清除氧自由基的作用。Objective： To study the effect of plantain seed polysaccharide （PSP） on oxidative stress in experimental alloxan-induced diabetes mice. Methods： The animal model was established with alloxan-induced mouse diabetes. Alloxan was injected intraperitoneally at a single dose of 150 mg·kg-1 for induction of diabetes. The model mice were divided into model group and PSP groups （P1, P2 and P3 groups）. The values of malondialdehyde （MDA）, nitrogen monoxide （NO） and nitrogen monoxide synthase （NOS）, suproxide （SOD）, catalase （CAT） and guguangantai peroxide enzyme hyperoxide mutase （GSH-PX） were detected by colorimetric reaction. The influence of PSP on the above biochemical indicators was observed. Results： Compared with the model group, the levels of blood serum/heart SOD, blood CAT, heart GSH-PX, kidney NO and blood serum NOS were no significant differences in the other groups except for P1 group. The levels of blood serum and heart MDA were significantly reduced but other biochemical indicators were significantly elevated. Conclusion： PSP has an effect of antioxidation and the action of free radical eliminations.

关 键 词：车前子 多糖类 糖尿病 实验性 小鼠 疾病模型 动物 氧化性应激 

分 类 号：R587.1[医药卫生—内分泌]