急性二氧化碳中毒大鼠不同氧疗效果评价  

Evaluation of different oxygen therapies on therapeutic effects in rats with acute carbon dioxide poisoning

作　　者：牛颖梅[1] 郝凤桐[1] 薛长江[1] 夏玉静[1] 周硕[1] 卢庆生 刘建中 张鹏 

机构地区：[1]首都医科大学附属北京朝阳医院职业病与中毒医学科,北京100020 [2]北京市疾病与预防控制中心

出　　处：《中华劳动卫生职业病杂志》2011年第3期202-205,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：科技部科研院所社会公益项目（200802020）

摘　　要：目的研究急性二氧化碳中毒大鼠应用不同氧疗的效果，以筛选急性二氧化碳中毒现场最佳氧疗技术。方法健康雄性SD大鼠60只，随机分为正常对照组、二氧化碳染毒组、高压氧治疗组（压力为2ATA、氧浓度100％）、高浓度常压吸氧治疗组（氧浓度50％）、低浓度常压吸氧治疗组（氧浓度33％）。以二氧化碳吸入制作急性二氧化碳中毒大鼠模型，给予不同方式氧疗后，检测各组大鼠动脉血pH、氧分压（PO2）和二氧化碳分压（PCO2），取肺组织和脑组织，观察病理变化。结果二氧化碳染毒组动脉血pH（7．31±0．06）和P02[（68．50±15．02）mmHg]比正常对照组[pH（7．42±0．02）和PO2（92．83±8．27）mmHg]低，PCO2[（71．66±12．10）mmHg]比正常对照组[（48．25±2．59）mmHg]高，差异均有统计学意义（P〈0．05）；高压氧治疗组、高浓度常压吸氧治疗组、低浓度常压吸氧治疗组动脉血pH（分别为7．37±0．02、7．39±0．03、7．38±0．02）和PO2[分别为（82．25±12．98）、（84．75±11．24）、（83．75±16．77）mmHg]比二氧化碳染毒组高，PCO2[分别为（52．25±4．95）、（51．75±4，82）、（52．66±5．61）mmHg]比二氧化碳染毒组低，差异均有统计学意义（P〈0．05）；各氧疗组动脉血pH、PO2和PCO2与正常对照组的差异无统计学意义（P〉0．05）；各氧疗组之间动脉血pH、PO2和PCO2的差异无统计学意义（P〉0．05）。病理大体解剖可见，二氧化碳染毒组大鼠肺脏可见大面积点、片状淤血，高浓度和低浓度常压吸氧治疗组的大鼠肺脏淤血情况较染毒组有所减轻，高压氧治疗组大鼠肺脏外观未见明显异常。光学显微镜下可见，二氧化碳染毒组大鼠肺组织出现弥漫性出血和渗出，高浓度和低浓度常压吸氧治疗组大鼠的肺组织出血和渗出情况较染毒组有所减轻，高压氧治疗组大鼠肺组织仅有轻微的�Objective To study therapeutic effects by using different oxygen therapies in rats with acute carbon dioxide poisoning, to select the best oxygen therapy technology for patients with acute carbon dioxide poisoning on the spot. Methods Sixty healthy male Sprague-Dawley rats were randomized into normal control group, carbon dioxide exposure group, hyperbaric oxygen treatment group （pressure 2 ATA, FIO2100% ）,high concentration of atmospheric oxygen treatment group （FIO2 50%）,low concentration of atmospheric oxygen treatment group（FiO2 33%）. After treated with different oxygen in rats with acute carbon dioxide poisoning, arterial pH, PO2 and PCO2 of rats were detected, in addition observe pathological changes of lung tissue and brain tissue. Results The arterial pH （7.31±0.06） and PO2 [（68.50±15.02）mm Hg] of carbon dioxide exposure group were lower than those of control group [pH （7.42±0.02） and PO2（92.83±8.27） mm Hg],PCO2[（71.66±12.10）mm Hg] was higher than that of control group[（48.25±2.59）mm Hg]（P〈0.05） ; the arterial pH （hyperbaric oxygen treatment group 7.37 ±0.02, high concentration of atmospheric oxygen treatment group 7.39±0.03, low concentration of atmospheric oxygen treatment group 7.38±0.02） and PO2 of oxygen treatment groups [hyperbaric oxygen treatment group, high concentration of atmospheric oxygen treatment group, low concentration of atmospheric oxygen treatment group were （82.25±12.98）, （84.75±11.24）, （83.75 ±16.77）mm Hg, respectively] were higher than that of carbon dioxide exposure group,PCO2 ]hyperbaric oxygen treatment group, high concentration of atmospheric oxygen treatment group, low concentration of atmospheric oxygen treatment group were （52.25±4.95）, （51.75±4.82）, （52.66±5.61 ）ram Hg, respectively] was lower than that of carbon dioxide exposure group （P〈0.05） ;there was no significant difference of the arterial pH, PO2 and PCO2 between oxygen treatment groups and control group （P〉0.05）; there w

关 键 词：二氧化碳 氧吸入疗法 高压氧 

分 类 号：R5[医药卫生—内科学]