冷应激对大鼠血管平滑肌细胞钙通道表达的影响  

Effect on expression of vascular smooth muscle cell calcium channel in rats exposed in low temperature

作　　者：杨冬梅[1] 张淑华[1] 葛郁芝[1] 王云霞[1] 刘燕锋[1] 吴志婷[1] 

机构地区：[1]江西省人民医院,江西省心血管病研究所,南昌330006

出　　处：《中华劳动卫生职业病杂志》2011年第3期224-226,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家科技支撑计划项目（2008BAI68B02）

摘　　要：目的观察冷应激对大鼠血管平滑肌细胞钙通道α1C亚基表达的影响。方法建立冷应激高血压模型。每2周测量大鼠的血压。24只健康成年sD大鼠随机分为对照组（6只）和寒冷暴露组（18只），寒冷暴露组大鼠分别于暴露的第2、4、6周处死，应用半定量反转录一聚合酶链反应（RT—PCR）检测主动脉钙离子通道α1C亚基的表达。结果寒冷暴露组大鼠血压从第2周开始升高，2周时冷应激大鼠模型血压为（102．8±2．25）mmHg，对照组大鼠血压为（89．2±3．73）mmHg，差异有统计学意义（P〈0．05）；4、6周时冷应激大鼠模型血压分别为（114．5±4．21）、（121．9±3．03）mmHg，与对照组比，差异有统计学意义（P〈0．01）。寒冷暴露组血管平滑肌钙离子通道α1C亚基mRNA水平升高，2、4、6周冷应激大鼠模型钙离子通道α1C亚基mRNA分别为0．21±0．02、0．33±0．05、0．57±0．08，与对照组（0．13±0．02）比，差异有统计学意义（P〈0．01）。钙通道α1C亚基mRNA的相对表达量与血压呈高度相关（r=0．86，P〈0．01）。结论反复寒冷暴露能诱发冷应激性高血压，导致血管平滑肌细胞上钙通道α1C亚基表达水平升高。Objective To observe the expression of vascular smooth muscle cells calcium channel α1C subunit （LTCCα1C） in rats exposed in low temperature. Methods Cold-induced hypertension was established and blood pressure was measured every two weeks. The mRNA expression of L type calcium channel α1C was determined by RT-PCR. Results The blood pressure of the rats exposed to cold environment increased. The blood pressure of experimental groups [（102.8±2.25）mm Hg] began to increase from the first two weeks, compared with the control group[（89.2±3.73 ）mm Hg], there were significant difference （P〈0.05）. The blood pressure of experimental groups were（ 114.5±4.21 ）, （121.9±3.03）mm Hg respectively at 4, 6 weeks. Compared with the control group, the expression of LTCCα1C mRNA of the cold exposure group increased significantly （P〈0.01）. There was a significant correlation between the expression of LTCCα1C mRNA and the blood pressure of the rats（r=0.86,P〈0.01 ）. Conclusion Repeated cold exposure can establish cold-induced hypertension, and the level of vascular smooth muscle cells LTCCα1C expression increase.

关 键 词：冷 高血压 钙通道 肌 平滑 血管 基因表达 

分 类 号：R544[医药卫生—心血管疾病]