黄芩甙对肝损伤小鼠肝抗氧化功能影响的研究  

Effects of Baicalin on Anti-oxidation Function in Liver of Liver Injury Mice

作　　者：龚争[1] 程郁昕[1] 章敏[1] 宋婷婷[1] 吕锦芳[1] 宁康健[1] 

机构地区：[1]安徽科技学院动物科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2011年第1期5-8,共4页Journal of Anhui Science and Technology University

基　　金：安徽省科技厅年度重点项目(06023087C)

摘　　要：目的:观察黄芩甙注射液对四氯化碳(CCl4)致急性肝损伤小鼠肝抗氧化功能的影响。方法:50只小鼠随机分为5组,每组10只,分别为对照组、模型组、黄芩甙注射液低、中、高(0.25 g/kg、0.50 g/kg、1.00g/kg)剂量组。用CCl4制备小鼠急性肝损伤模型,测定小鼠体重变化,肝指数,肝超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,谷胱甘肽(GSH)及丙二醛(MDA)含量。结果:与对照组及模型组比较,黄芩甙各剂量组小鼠试验前后的体重及肝指数差异不显著(P>0.05)。与模型组比较,低剂量组小鼠肝SOD、GSH-Px活性及MDA含量差异不显著(P>0.05),而GSH含量显著升高(P<0.05);中、高剂量组SOD、GSH-Px活性极显著提高(P<0.01),GSH含量极显著升高(P<0.01),MDA含量极显著降低(P<0.01)。结论:黄芩甙能显著增强急性肝损伤小鼠肝SOD、GSH-Px活性,提高GSH含量,降低MDA含量,并表现正向的量效关系。Objective： To observe the effects of baicalin injection on anti-oxidation function in mice liver of the acute liver injury induced by carbon tetrachloride（CCl4）.Method： 50 mice were randomly divided into five groups,10 mice every group,while the groups were the control group,the model group,and the baicalin groups at low,middle and high doses（0.25g/kg,0.50g/kg,1.00g/kg） respectively.The acute liver injury model of mice were induced by CCl4,the body weight,the liver index,the activity of SOD and GSH-Px,and the content of GSH and MDA of mice were determined.Results： There were not significant differences（P0.05） of the body weight and the liver index of mice among five groups.Comparing with the model group,the activity of SOD and GSH-Px,and the content of MDA in liver of mice,which in the baicalin group at low dose,were not significantly differences（P0.05）,but the content of GSH increased significantly（P0.05）.Comparing with the model group,the activity of SOD and GSH-Px in liver of mice,which in the baicalin groups at middle and high doses,increased very significantly（P0.01）,the content of GSH increased very significantly（P0.01）,and the content of MDA decreased very significantly（P0.01）.Conclusion： Baicalin could significantly increase the activity of SOD and GSH-Px in liver of mice with acute liver injury,increase the content of GSH,and decrease the content of MDA,and there was a positive relationship between dose and effect.

关 键 词：黄芩甙 小鼠 肝损伤 超氧化物歧化酶 谷胱甘肽过氧化物酶 谷胱甘肽 丙二醛 

分 类 号：Q485[生物学—生理学]