姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响  

Effect of Curcumin on Proliferation and Apoptosis of Human Nasopharyngeal Carcinoma CNE-2Z Cells

作　　者：范德生[1] 甄蕾[2] 孙宁[3] 

机构地区：[1]上海中医药大学附属曙光医院病理科,上海200021 [2]同济大学医学院 [3]广东医学院病理学教研室

出　　处：《肿瘤防治研究》2011年第3期254-256,共3页Cancer Research on Prevention and Treatment

基　　金：广东省教育厅自然科学研究基金资助项目(GK0404)

摘　　要：目的探讨姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响及其相关的分子机制。方法体外培养的CNE-2Z细胞经不同剂量姜黄素处理后,用MTT法观察对细胞增殖的影响,流式细胞术检测细胞周期和凋亡率的改变,Western blot检测姜黄素处理后相关蛋白Bcl-2、Bax表达水平的变化。结果姜黄素对CNE-2Z细胞的生长和增殖都具有一定程度的抑制作用,阻断细胞于S期和G2/M期。姜黄素处理后CNE-2Z细胞凋亡抑制基因Bcl-2蛋白表达减少,同时促凋亡基因Bax蛋白表达增加。结论姜黄素可抑制CNE-2Z细胞增殖并促进其凋亡。Objective To study the effects of Curcumin on proliferation and apoptosis of human nasopharyngeal carcinoma cell CNE-2Z and its possible mechanism.Methods After treatment with Curcumin at various concentrations,the proliferative inhibition of CNE-2Z was observed by MTT assay.The cell cycle influence and cell apoptosis rate were detected by flow cytometry.The expression of Bcl-2 and Bax were detected by Western blot.Results Curcumin could inhibit the proliferation of human nasopharyngeal carcinoma cells CNE-2Z at a time and dose dependent pattern.The cells were blocked at G2/M and S phases.When treated with Curcumin for 24h,the percentage of cell apoptosis was increased.Bcl-2 was down-regulated whereas Bax was up-regulated.Conclusion Curcumin could inhibit the proliferation of CNE-2Z and induce cell apoptosis.

关 键 词：姜黄素 鼻咽癌 增殖 凋亡 BCL-2 BAX 

分 类 号：R793.63[医药卫生—临床医学]