ZK-1注射液抗大鼠肝纤维化作用的实验研究  

Experimental studies of glutathione compound injection on anti-hepatic fibrosis in rats

作　　者：罗胜勇[1] 叶寿山 马征[1] 李心伟[1] 贾德云 倪光玉 

机构地区：[1]安徽省医学科学研究院药理毒理研究所

出　　处：《中国临床药理学与治疗学》2011年第1期18-21,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察ZK-1注射液对N,N-二甲基亚硝胺诱导大鼠肝纤维化形成的抑制作用。方法:采用N,N-二甲基亚硝胺建立大鼠肝纤维化模型,血生化分析仪检测大鼠肝功能,酶联免疫吸附(ELISA)法测血清中透明质酸(HA)、转化生长因子β-1(TGFβ-1)含量,测定组织中羟脯氨酸(HYP)含量,并行肝脏组织病理学检查。结果:与模型组比较,ZK-1注射液可明显降低大鼠谷草、谷丙转氨酶水平,明显提高血清总蛋白和白蛋白含量;明显降低血清HA、TGFβ-1含量和肝组织中HYP的水平。组织病理学检查显示,对肝组织纤维化病变有一定程度的改善作用。结论:ZK-1注射液对N,N-二甲基亚硝胺诱导大鼠肝纤维化形成具有一定的抑制作用,其机制可能与抑制TGFβ-1产生有关。AIM ： To investigate the inhibitory action of glutathione compound injection on dimethylnitrosamine-induced hepatic fibrosis in rats. METHODS ： The hepatic fibrosis model in rats were established by injection of dimethylnitrosamin.The liver function,levels of hyaluronic acid（HA）,hepatic hydroxyproline（HYP）,transforming growth factor β-1（TGFβ-1） and the pathological change were detected. RESULTS ：Compared with model group,glutathione compound injection could markedly improve the liver function,significantly decreased the levels of HA,HYP and TGFβ-1,the pathological changes could also be alleviated. CONCLUSION ：Glutathione compound injection has protective effect against hepatic fibrosis,the possible mechanism might be related to decreasing the level of TGFβ-1.

关 键 词：N N-二甲基亚硝胺 透明质酸 羟脯氨酸 转化生长因子-Β1 

分 类 号：R965.1[医药卫生—药理学]