NF-κB、IκB在阿霉素肾病大鼠中的表达及大黄蛰虫丸的干预作用  

Expression of NF-κB and IκB in rats with adriamycin nephropathy and the role of Dahuangzhechong Wan

作　　者：陈继红[1] 周栋[1] 高坤[1] 何伟明[1] 王跃娟[1] 孙伟[1] 

机构地区：[1]江苏省中医院肾内科

出　　处：《中国临床药理学与治疗学》2011年第1期22-26,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨NF-κB、IκB在阿霉素肾病大鼠中的表达及大黄蛰虫丸的干预作用。方法:SD大鼠一次性尾静脉注射阿霉素5 mg/kg,随机分为模型组(n=30)和大黄蛰虫丸组(n=15),另以8只正常大鼠作为对照组(正常组),进行对比观察。于实验第7天、14天,各处死4只模型组大鼠,留取肾脏标本待测。实验第28天,大鼠全部处死,观察大鼠超微结构的改变,应用免疫荧光方法及免疫组织化学方法定位及定量分析检测NF-κB、IκB-α的表达变化。结果:与正常对照组比较,模型组大鼠肾小球足细胞结构受到损害,足细胞足突融合,肾小球内NF-κB表达增加[(2.491±0.551)×106],IκB-α表达下调[(0.947±0.631)×106],大黄蛰虫丸可明显抑制阿霉素肾病大鼠肾小球内NF-κB的表达[(1.165±0.374)×106],减轻足细胞足突融合。结论:大黄蛰虫丸可能通过影响NF-κB、IκB的表达而对足细胞起保护作用。AIM ： To investigate the expression of NF-κB and IκB in rats with adriamycin nephropathy and the intervention of Dahuangzhechong Wan. METHODS ： The rats were injected with 5 mg/kg adriamycin solution from vena caudalis,then the rats were divided into two groups randomly： Adriamycin nephropathy group（n=30） and Dahuangzhechong Wan（DHZCW） group（n=15）,and the other 8 rats were selected as the control group.On the 7th day,the 14th day,4 rats from the model group were sacrificed,the renal tissue was reserved for detection.On the 28th day,the rats were all put to death.The changes of the renal tissue ultrastructure were observed.Immunofluorescence,immunohistochemistry and quantitive analysis were used to detect the expression of NF-κB and IκB-α. RESULTS ：Compared with the control rats,the expression of NF-κB in model group increased markedly[（2.491 ± 0.551）×10 6],accompanying with the down-regulation of IκB-α[（0.947 ± 0.631）×10 6],and the podocyte was injuried.DHZCW could suppress the expression of NF-κB[（1.165 ± 0.374）×10 6 ],lessen the podocyte lesion. CONCLUSION ： DHZCW has the protection effect on podocyte by influencing the expression of NF-κB and IκB.

关 键 词：大黄蛰虫丸 NF-ΚB IΚB 阿霉素肾病 

分 类 号：R965.1[医药卫生—药理学]