人胚胎脑室脉络丛上皮细胞表达神经干细胞分子标志  

Human embryo choroid plexus epithelial cells express neural stem cell markers

作　　者：张小燕[1] 刘胜勇[2] 何其华[1] 陆爱丽[1] 王珂[2] 张卫光[2] 沈丽[1] 

机构地区：[1]北京大学医学部基础医学院细胞生物学系 [2]北京大学医学部基础医学院解剖学与组织胚胎学系,北京100191

出　　处：《解剖学报》2011年第2期201-204,共4页Acta Anatomica Sinica

摘　　要：目的探讨人12周龄胚胎脑脉络丛上皮细胞是否具有神经干细胞的生物学特性。方法制备室管膜/室管膜下区和纹状体脑切片和脉络丛组织铺片,采用免疫荧光染色,在激光扫描共焦显微镜下观察结果,采集图像数据。结果人12周龄胚胎脑室脉络丛上皮细胞表达神经干细胞分子标志CD133、Nestin和Sox2。神经干细胞的分子标志在脉络丛上皮细胞的分布具有一定极性,未见共定位分布。室管膜上皮细胞面向脑室腔面的细胞顶端微绒毛也表达CD133,而室管膜下区和纹状体区可见极少数CD133阳性标记细胞。结论 12周龄脑室脉络丛上皮细胞已定向分化,不仅提供维持神经干细胞的微环境,还具有神经干细胞的生物学特性。Objective In order to investigate whether the 12-week-old embryo brain ehoroid plexus epithelial cells have neural stem cell biological properties. Method Preparation of ependymal / subventricular zone （SVZ） and striatum brain sections and whole-mound sections of choroid plexus tissue was made by immunofluorescence staining, and under lasers canning confocal microscopy to observe the results and collect images. Results Twelve-week-old embryo choroid plexus epithelial cells express neural stem cell markers CD133, Nestin and Sox2. Neural stem ceils markers were in polarity distribution in the choroid plexus epithelial cells, there was no colocalization. A part of microvilli in the apical surface of the choroid plexus epithelial cells facing the ventricular cavity also expressed CD133, while the SVZ and striatum showed a very small number of CD133 positive cells. Conclusion Twelve-week-old embryo brain choroid plexus epithelial cells are destined to differentiate, providing not only the micro-environment for maintenance of neural stem cells in niche but also have properties of neural stem cells.

关 键 词：脉络丛上皮细胞 室管膜 神经干细胞 免疫荧光染色 胚胎 人 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]