门静脉高压症猪肝动脉的结构重建  

Structure remodeling of swine hepatic artery in portal hypertension

作　　者：贺细菊[1] 于明华[1] 李文春[1] 张兴华[1] 黄铁柱[1] 

机构地区：[1]郧阳医学院生物力学实验室,湖北十堰442000

出　　处：《解剖学报》2011年第2期241-244,共4页Acta Anatomica Sinica

基　　金：湖北省教育厅科学技术研究项目(D200524002;D20102103)

摘　　要：目的建立猪门静脉高压症模型,探讨门静脉高压症时肝动脉的结构重建。方法猪以四氯化碳、苯巴比妥、乙醇配合高脂、低蛋白、低胆碱饮食进行混合饲养。通过脾静脉插管测压,取肝动脉常规石蜡包埋、切片,用HE法、Weigert法、Aniline blue法,Organge G法分别染组织结构、弹性纤维、胶原纤维和平滑肌,用计算机图像分析系统定量分析肝动脉的几何形态及显微成分。结果实验组门静脉压为(4.17±1.03)kPa,明显大于对照组(1.51±0.79)kPa;实验组肝动脉的内膜与中膜增厚,管壁增厚,管径增粗,胶原纤维的百分含量显著增加,弹性纤维的百分含量显著降低,平滑肌细胞核的数密度和面密度增加,胶原纤维与弹力纤维比值增加。结论门静脉高压症时,与血液动力学改变相适应,肝动脉的几何形态与显微结构成分均发生了显著变化。Objective To establish a reliable swine portal hypertension model and explore the morphological properties of hepatic arteries. Methods Liver cirrhosis and portal hypertension were induced by carbon tetrachlorideand fenobarbital （n = 8）, 4 pigs served as the control, the hepatic arteries were collected and serially sectioned. The tissue structure, elastic fiber, collagen fiber and smooth muscle were stained by HE, Weigert, Aniline blue and Orange G respectively. The morphological data of hepatic arteries were obtained by the computer image analysis system. Results The portal vein pressure of experimental group was （4. 17 ＋ 1.03 ） kPa, that of the control group was （ 1.51 ＋ 0. 79 ） kPa. The intima and media thickness of hepatic artery was obviously larger than that of the control （ P 〈 0. 05 ）. The wall thickness and the diameter of hepatic artery were obviously larger than that of the control （ P 〈 0.01 ）, the relative percentage of collagen fibers（C） was significantly larger than that of the control （P 〈 0. 01 ）, the relative percentage of elastic fibers （ E ） decreased significantly （ P 〈 0. 05 ）, the ratio of C and E increased significantly. Conclusion The hepatic artery has suffered extensive morphological remodeling while portal hypertension, and in return affected the hepatic artery buffer response.

关 键 词：门静脉高压症 肝动脉 显微结构 组织化学 猪 

分 类 号：R657.34[医药卫生—外科学]