层黏连蛋白受体在小鼠睾丸和附睾中的表达  

Expression of laminin receptor in testis and epididymis of adult male mice

作　　者：李善妮[1] 王行明[1] 孙双午[1] 王璐[1] 黄霁[2] 滕春波[3] 丁乃峥[1] 

机构地区：[1]中南大学生物科学与技术学院,长沙410013 [2]湖南师范大学树达学院,长沙410081 [3]东北林业大学生命科学学院,哈尔滨150040

出　　处：《解剖学报》2011年第2期283-287,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30400039);国家大学生创新性实验计划项目(YA07070);湖南省青年骨干教师培养计划(2009);湖南省普通高等学校教学改革项目(2009029);中南大学研究生教育教改研究项目(2960-71131410015);中南大学大学生创新性实验计划项目(YB07109)

摘　　要：目的探讨层黏连蛋白受体(LAMR1)在小鼠睾丸和附睾中的表达。方法收集3只正常成年昆明小鼠睾丸和附睾。采用原位杂交和免疫组织化学方法,检测LAMR1 mRNA及蛋白在成年小鼠睾丸和附睾中的分布。结果 LAMR1 mRNA在附睾头和附睾尾表达最强;在睾丸生精细胞中也有表达。免疫组织化学结果显示,LAMR1蛋白从附睾头到附睾尾部表达逐渐增强,在睾丸生精细胞和附睾精子上也检测到了LAMR1蛋白的表达。结论 LAMR1在成年小鼠睾丸和附睾中具有区域特异性表达模式,并存在于附睾管精子中,表明其可能与附睾的某些功能有关,并可能参与精子发生和成熟等过程。Objective To provide a basis of further studying functional significance of the laminin receptor 1 （LAMR1） in spermatogenesis, sperm mature and fertilization, the expression of the LAMR1 in testis and epididymis of adult male mice was studied. Method Testis and epididymis from three normal Kunming white male mice were collected. In situ hybridization and immunohistochemistry were applied to detect the expression of the LAMR1 in testis and epididymis of adult male mice. Results The result of in situ hybridization showed that LAMR1 mRNA expressed in the epididymis caput and cauda at high levels, and also was detected in spermatogenic cell and spermatids. The immunohistoehemistry result showed that the intensities of immunohistochemical staining of LAMR1 tended to strengthen from epididymal caput to epididymal cauda, and also was detected in spermatogenic cells, spermatids and epididymis spermatozoa. Conclusion LAMR1 has a specific distribution on testis and epididymis of adult mice, suggesting that it is likely to have some relations to the spermatogenesis and function of epididymis. The high expression in epididymis cauda spermatozoa supports the hypothesis that LAMR1 plays a role during fertilization.

关 键 词：层黏连蛋白受体 睾丸 附睾 原位杂交 免疫组织化学 小鼠 

分 类 号：Q492[生物学—生理学] Q291