猪干扰素-γ基因多态性及与早期生长、繁殖性能关联性分析  

Studies on Polymorphisms of Interferon-γ Gene and Its Association with the Early Growth Performances and Reproduction in Porcine

作　　者：王大力[1,2] 赵伟[1] 孙海涛[2] 孙博兴[1] 黄大鹏[2] 赵志辉[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]黑龙江八一农垦大学动物科技学院,大庆163319

出　　处：《畜牧兽医学报》2011年第3期335-342,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：吉林省科技发展计划项目(20080208);吉林大学基本科研业务费科学前沿与交叉学科创新项目(421033476201)

摘　　要：为研究IFN-γ基因作为猪抗病育种中一种分子标记的可能性,本试验采用PCR-SSCP技术检测包括军牧1号白猪、杜洛克猪、大白猪3个品种共481头个体的IFN-γ基因全序列单核苷酸多态性,并对其与早期生长性能、繁殖性能的关联性进行分析。结果表明,在猪IFN-γ基因内含子1、内含子3、外显子4内各检测到一个突变位点,分别为T825C、T2370C、G5301A;在T825C位点AB基因型个体的70日龄体质量显著高于AA和BB型个体(0.01<P<0.05),AA和BB型间差异不显著(P>0.05),断奶至70日龄日增体质量AB基因型个体极显著高于AA和BB型个体(P<0.01),AA和BB型间差异不显著(P>0.05);T2370C、G5301A位点的多态性对早期生长性能的影响不显著(P>0.05)。军牧1号白猪G5301A位点EF基因型个体的产仔数、产活仔数显著高于EE型个体(0.01<P<0.05)。T825C、T2370C位点各基因型间的产仔数、产活仔数、断奶仔猪数、初生窝质量、21日龄窝质量差异均不显著(P>0.05)。研究结果提示:猪IFN-γ基因T825C位点多态性对仔猪70日龄体质量、断奶至70日龄日增体质量有影响;G5301A位点多态性对母猪的产仔数、产活仔数有影响。To study the possibility of Interferon-γ（IFN-γ） gene as a genetic marker in porcine resistance breeding,the single nucleotide polymorphisms（SNPs） of the IFN-γ gene were detected in 481 pigs including three breeds of Junmu-1 White,Yorkshire and Duroc pigs.The correlations between SNPs and the early growth performances of piglet and reproduction of sow were analyzed.The results showed that three mutation sites were determined in intron 1,intron 3 and exon 4 of IFN-γ gene.The mutation sites were T825C,T2370C and G5301A.The results showed that 70 d weight of piglets with genotype AB（T825C site） was higher than that of piglets with genotype AA and BB（0.01P0.05）,but the difference between individuals with AA and BB genotypes was not significant（P0.05）.The average daily gain（ADG） of weaning-to-70 d piglet with AB genotype was higher than that of piglets with AA and BB genotypes（P0.01）,but difference between individuals with genotype AA and BB was not significant（P0.05）.Difference of birth weight,weaning weight and birth-to-weaning weight among pigs in all genotypes were not significant（P0.05）.The effect of polymorphism at the sites of T2370C and G5301A on the early growth performance of pigs were not significant（P0.05）.The litter size,litter size born alive of pigs with genotype EF（G5301A） was higher than that of pigs with genotype EE in the Junmu-1 White pigs（0.01P0.05）.The difference of the litter size,litter size born alive,litter size at weaning,litter weight,litter size at 21 days among pigs with all genotypes at the sites of T825C and T2370C were not significant（P0.05）.In the conclusion,we can found some effects of SNPs（T825C） on 70 d weight and ADG of weaning-to-70 d,and some effects of SNPs（G5301A） on litter size and litter size born alive of sows.

关 键 词：干扰素-γ基因 多态性 早期生长性能 繁殖性状 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]