817肉杂鸡肉瘤组织分离出A、J亚型禽白血病病毒  

The Separation of Subgroup A and J ALV in Soft Tissue Sarcomas of "817" Broiler Hybrids

作　　者：刘绍琼[1] 王波[1] 张振杰[1] 王健[1] 孙淑红[1] 崔治中[1] 

机构地区：[1]山东农业大学动物医学院,泰安271018

出　　处：《畜牧兽医学报》2011年第3期396-401,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家公益性行业(农业)科技专项(20080319);山东省科技发展计划(2010GNC10924)

摘　　要：山东某地饲养的36日龄817肉杂鸡生长迟缓并发生颈部肉瘤。取肉瘤组织上清液接种CEF,培养12d,用IFA试验进行病毒鉴定。检测结果表明,从该肉瘤组织分离到A亚型与J亚型禽白血病病毒(ALV-A、ALV-J),命名为SD1005株。取肉瘤组织上清液人工感染817肉杂鸡与SPF鸡进行动物回归试验,结果复制出同样肉瘤。用复制出的新鲜肉瘤分别制备肉瘤细胞与肉瘤组织触片,IFA试验再次验证出ALV-A与ALV-J均为阳性,而且发现ALV-A与ALV-J分别共感染同一个组织细胞。同时,通过PCR方法用针对ALV-A、ALV-J的2对引物扩增并鉴定出肉瘤组织中含有ALV-A、ALV-J。对扩增的ALV-J囊膜糖蛋白(env)基因1 710bp片段进行的遗传变异分析结果显示,SD1005株与14株国内外参考株之间的同源性为88.5%～94.0%,其中与来自白羽肉鸡的美国参考株0661(AF247566)、ADOL-Hc1(AF097731)的同源性最高,分别为94.0%、93.8%。本研究显示,来自817肉杂鸡的肉瘤组织上清液接种鸡可诱发急性肉瘤,其中既检出ALV-A,又检出ALV-J,但急性肉瘤由单个病毒诱发还是与共感染相关有待于进一步研究。The jugular soft tissue sarcomas were obtained from ＂817＂ broiler hybrids.The tissue and cell lysis solution was prepared and inoculated in CEF cells for 12 days′ culture,as well as ＂817＂ Broiler Hybrids and SPF chickens,ALV-A and ALV-J viruses were identified by IFA from the culture and then to be named as SD1005.The duplicate soft tissue sarcomas of ＂817＂ Broiler Hybrids were prepared to culture and touch the piece,and then ALV-A and ALV-J were identified by IFA.In the meantime,ALV-A and ALV-J were discovered co-infection in the same cell.ALV-A and ALV-J were identificated with two pairs of primers toward ALV-A and ALV-J by PCR respectively.The result of autogenous variation analysis of env gene（1 710 bp） to ALV-J indicated that the homology between SD1005 and 14 reference strains at home and abroad was 88.5%-94.0%,the highest homology of which with U.S.A strains 0661 and ADOL-Hc1 were 94.0%,93.8%.The present results indicated that there were mixed viruses in the soft tissue sarcomas from ＂817＂ broiler hybrids,with acute and high tumorigenicity.However,whether it was caused by ALV-A and ALV-J and the underlying mechanism of its tumorigenicity needs to be investigated further.

关 键 词：A、J亚型禽白血病病毒 共感染 分离鉴定 

分 类 号：S852.659.3[农业科学—基础兽医学]