鸡β-防御素-1对鸡传染性法氏囊病病毒VP2基因DNA疫苗的免疫佐剂作用  

The Adjuvant Effects of Chicken β-defensin-1 on Infectious Bursal Disease Virus VP2 Gene DNA Vaccine

作　　者：张辉华[1] 杨小梅[2] 谢青梅[2] 马静云[2] 罗艳娜[1,2] 曹永长[3] 毕英佐[2,3] 

机构地区：[1]佛山科技学院生命科学院,佛山528231 [2]华南农业大学动物科学学院,广州510642 [3]中山大学生命科学学院,广州510275

出　　处：《畜牧兽医学报》2011年第3期402-408,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：广东省自然科学基金项目(5300444);广东省科技厅农业科技攻关项目(2006B20101015)

摘　　要：本文旨在研究鸡β-防御素-1(AvBD1)对鸡IBDVVP2基因DNA疫苗的免疫佐剂作用。通过基因工程技术,构建真核表达质粒pcDNA3.1(+)-AvBD1与pcDNA3.1(+)-VP2。14日龄岭南黄肉鸡随机分成5组,分别免疫PBS、空质粒载体pcDNA3.1(+)、重组质粒pcDNA3.1(+)-VP2、重组质粒pcDNA3.1(+)-AvBD1与pcD-NA3.1(+)-VP2联合免疫、IBD弱毒苗。收集免疫后不同时期的外周血血清样本,用ELISA方法检测血清中IB-DV VP2特异性抗体水平,通过流式细胞仪检测CD3+、CD4+与CD8+T淋巴细胞的含量。结果表明,联合免疫组(即pcDNA3.1(+)-AvBD1与pcDNA3.1(+)-VP2共同免疫)VP2特异性抗体水平显著高于其他组;单独免疫质粒pcDNA3.1(+)-VP2组VP2特异性抗体水平与免疫IBD弱毒苗组相当,显著高于PBS组与空质粒载体pcD-NA3.1(+)组。在二免后第7天,联合免疫组外周血中CD3+、CD4+与CD8+T淋巴细胞的含量也显著高于单独免疫质粒pcDNA3.1(+)-VP2组,其它时间无显著性差异。IBDV攻毒后的保护率也是联合免疫组高于单独免疫pcDNA3.1(+)-VP2组。这些结果表明,鸡AvBD1可以作为一种免疫佐剂用于增强IBDVVP2基因DNA疫苗的免疫力。The aim of present study was to investigate the adjuvant effects of chicken β-defensin-1（also named avian β-defensin-1,AvBD1） on DNA vaccine encoding chicken infectious bursal disease virus（IBDV） VP2 gene.The eukaryotic expression vectors pcDNA3.1（＋）-AvBD1 and pcDNA3.1（＋）-VP2 were constructed.Five groups of 14-day-old chickens were intramuscularly injected with PBS,empty vector pcDNA3.1（＋）,pcDNA3.1（＋）-VP2,a mixtures of pcDNA3.1（＋）-AvBD1 and pcDNA3.1（＋）-VP2,and IBD attenuated vaccine,respectively.Serum of chicken in each group was collected from the peripheral blood at different time post inoculation,and VP2-specific antibody was measured by ELISA.Content of CD3＋,CD4＋ and CD8＋ T-lymphocytes in each group of chickens were tested by cytospectrometry.Results showed that VP2-specific antibody level of group immunized with a mixtures of pcDNA3.1（＋）-AvBD1 and pcDNA3.1（＋）-VP2 was the highest among all the groups.The VP2-specific antibody level of group immunized with pcDNA3.1（＋）-VP2,as well as group immunized with IBD attenuated vaccine,was significantly higher than that of the group immunized with PBS and empty vector pcDNA3.1（＋）.The percentages of CD3＋,CD4＋ and CD8＋ T-cell subtypes between groups of pcDNA3.1（＋）-VP2 and pcDNA3.1（＋）-VP2 combination with pcDNA3.1（＋）-AvBD1 was significantly different（P0.05）,the latter was higher,at 7 days post boost.The protection from IBD challenged by immunized chickens with DNA vaccines encoding IBDV VP2 gene alone was lower than that by immunized with a mixtures of pcDNA3.1（＋）-AvBD1 and pcDNA3.1（＋）-VP2.The results indicated that AvBD1 can be used as an adjuvant to enhance the IBDV VP2 DNA vaccine immunity.

关 键 词：鸡β-防御素-1(AvBD1) 免疫佐剂 IBDV DNA疫苗 

分 类 号：S852.659.4[农业科学—基础兽医学] S852.52[农业科学—兽医学]