小花棘豆产苦马豆素内生真菌的筛选与鉴定  

Screening and Identification of Swainsonsine-producing Endophytic Fungi from Oxytropis. flabra

作　　者：卢围[1] 路浩[1] 赵宝玉[1] 荣杰[1] 陈基萍[1] 王瑞[1] 高文超[1] 陈占莉[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2011年第3期429-436,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31072175)

摘　　要：通过对小花棘豆内生真菌的分离纯化,筛选产苦马豆素内生真菌,探讨小花棘豆内生真菌与其毒性成分苦马豆素的相互关系。本研究对采自内蒙古西部草地的6份小花棘豆样品进行内生真菌分离纯化,采用薄层色谱和气相色谱技术对内生真菌发酵液进行苦马豆素定性、定量检测,筛选产苦马豆素内生真菌;结合形态学和分子生物学方法进行内生真菌种属鉴定。从小花棘豆样品中分离得到6株内生真菌。经筛选,其中有2株产苦马豆素,编号为XJ-J-I和XJ-H-1,其发酵液中苦马豆素产量分别为4.209 2mg.L-1和16.733 8mg.L-1。鉴定结果表明,这2株内生真菌虽然形态学存在差异,但基因型相同,同属镰刀属内生真菌—层出镰刀菌(Fusarium prolifera-tum)。小花棘豆中存在产苦马豆素内生真菌。To screen and obtain swainsonsine-producing endophytic fungi in Oxytropis.flabra,explore the relationship between endophytic fungi and swainsonine toxin in Oxytropis.flabra.In this research,we isolated and purified endophytic fungi of 6 Oxytropis.flabra samples from the west Inner Mongolia.Swainsonine was detected by thin layer chromatography and gas chromatography in fermentation liquor of the isolated fungus,then screened the Swainsonsine-producing endophytic fungi.Combined colonial morphology and micro-morphology to identify the species of isolated fungus.Finally got 6 species of endophytic fungi through identification.2 of the 6 species endophytic fungi can produce swainsonine,named them XJ-J-1 and XJ-H-1 respectively.The Swainsonine yield was determined by gas chromatography,4.209 2 mg·L-1 in the fermentation liquor of XJ-J-1 and 16.733 8 mg·L-1 in the fermentation liquor of XJ-H-1.The 2 strains endophytic fungi have the same genotype but different phenotype,both of them are Fusarium proliferatum.The results show that the swainsonine-producing endophytic fungi can be isolated from the Oxytropis.flabra.

关 键 词：小花棘豆 内生真菌 苦马豆素 层出镰刀菌 疯草 

分 类 号：S852.66[农业科学—基础兽医学]