序贯培养液G1/G2对山羊核移植胚胎发育和质量的影响  

Effect of Sequential Media G1/G2 on Development and Quality of Goat SCNT Embryos

作　　者：刘军[1] 郑聪颖[1] 李长雷[1] 杜珊[1] 李亮亮[1] 马保华[1] 张涌[1] 

机构地区：[1]西北农林科技大学动物医学院农业部动物生理生殖和胚胎工程重点开放实验室,杨凌712100

出　　处：《畜牧兽医学报》2011年第3期442-447,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家转基因生物新品种培育科技重大专项(2008ZX08007-004)资助

摘　　要：为了优化山羊核移植胚胎体外培养体系,提高核移植效率,本研究检测了山羊体细胞核移植(SCNT)胚胎在序贯培养液G1/G2中的发育率和囊胚细胞凋亡,以及核移植胚胎移植后的妊娠率,以传统mSOF-FBS培养液作为对照组,评估序贯培养液G1/G2支持山羊核移植胚胎的发育能力。结果显示,与对照组相比,G1/G2组的囊胚发育率差异不显著((27.7±3.1)%vs(25.3±1.0)%,P>0.05),囊胚细胞数和囊胚细胞凋亡率显著降低(分别为(93.2±4.5)vs(109.1±6.2)和(4.9±0.2)%vs(11.3±0.1)%,P<0.05),但移植后的妊娠率显著增高(21.4%vs 8.0%,P<0.05)。结果表明,与传统的培养液mSOF-FBS相比,序贯培养液G1/G2能更好地支持山羊核移植胚胎的发育。The aims of this study were to investigate the effects of sequential media G1/G2 and modified synthetic oviductal fluid（mSOF） culture systems on developmental competence,cell apoptosis and the pregnant rate of goat somatic cell nuclear transfer（SCNT） embryos.Goat NT embryos were cultured as following：（1） 72 h in G1 then 120 h in G2（group G1G2）,both the culture media supplemented with 0.8% BSA（bovine serum albumin）;（2） 72 h in mSOF supplemented with 0.8% BSA then in mSOF supplemented with 10% FBS（fetal bovine serum） for 120 h（group mSOF-FBS）.The results indicated that there was no significant difference between the sequential media G1/G2 and mSOF-FBS in blastocyst rates（（27.7±3.1）% vs（25.3±1.0）%,P0.05）.Both of total cell number of blastocysts and apoptotic nuclei in blastocyst from G1/G2 were significantly lower than that in mSOF-FBS（（93.2±4.5） vs（109.1±6.2） and（4.9±0.2）% vs（11.3±0.1）%,respectively,P0.05）.However,the pregnant rates on Day 30 in group G1/G2 were higher than those in group mSOF-FBS（21.4% vs 8.0%,P0.05）.In conclusion,compared to mSOF-FBS,the sequential media G1/G2 could better support the development of goat somatic cell nuclear transfer embryos.

关 键 词：山羊 胚胎培养 体细胞核移植 凋亡 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]