Effects of cotransplantated Schwann cells and neural stem cells in a rat model of Alzheimer's disease  

作　　者：Yan Zhan Dihui Ma Yu Zhang 

机构地区：[1]Department of Neurology, Affiliated Hospital of Hebei University, Baoding 071000, Hebei Province, China [2]Department of Neurology, First Clinical Hospital of Jilin University, Changchun 130021, Jilin Province, China

出　　处：《Neural Regeneration Research》2011年第4期245-251,共7页中国神经再生研究（英文版）

基　　金：the Science and Technology Development Foundation of Jilin Province, No. 200505204, 200705129

摘　　要：Schwann cells （SCs） are significantly better at promoting neural stem cell （NSCs） proliferation, differentiation and synaptic formation when cocultured with NSCs in vitro, compared with cultured in a single nerve growth factor. The present study transplanted NSCs and SCs into the brain of a rat model of Alzheimer＇s disease to investigate the effect of cotransplantation. Results show transplantation of both NSCs alone and NSCs ＋ SCs significantly promoted learning and memory functions in Alzheimer＇s disease rats, decreased glial fibrillary acidic protein and calcium binding protein S100β expression, but increased expression of the cholinergic neuron marker choline acetyl transferase mRNA. The effect of NSCs ＋ SCs cotransplantation was, however, more significant. NSCs and SCs cotransplantation significantly reduced the number of astrocytes and increased cholinergic neurons, facilitating the recovery of learning and memory function, compared with NSCs transplantation alone.Schwann cells （SCs） are significantly better at promoting neural stem cell （NSCs） proliferation, differentiation and synaptic formation when cocultured with NSCs in vitro, compared with cultured in a single nerve growth factor. The present study transplanted NSCs and SCs into the brain of a rat model of Alzheimer＇s disease to investigate the effect of cotransplantation. Results show transplantation of both NSCs alone and NSCs ＋ SCs significantly promoted learning and memory functions in Alzheimer＇s disease rats, decreased glial fibrillary acidic protein and calcium binding protein S100β expression, but increased expression of the cholinergic neuron marker choline acetyl transferase mRNA. The effect of NSCs ＋ SCs cotransplantation was, however, more significant. NSCs and SCs cotransplantation significantly reduced the number of astrocytes and increased cholinergic neurons, facilitating the recovery of learning and memory function, compared with NSCs transplantation alone.

关 键 词：Schwann cells neural stem cells CO-TRANSPLANTATION ETHOLOGY glial fibrillary acidic protein S100Β choline acetyl transferase mRNA Alzheimer＇s disease 

分 类 号：Q813[生物学—生物工程] Q421